Liu Yu, Wang Chen, Xu Wenqing, Fan Ruike, Wu Zhigang, Dai Lishang
School of Traditional Chinese Medicine, Wenzhou Medical University, Wenzhou, 325035, P.R. China.
The Second Clinical School of Medicine, Wenzhou Medical University, Wenzhou, 325035, P.R. China.
BMC Plant Biol. 2025 Feb 20;25(1):233. doi: 10.1186/s12870-025-06132-6.
Curcuma wenyujin, a perennial herb of the ginger family, is renowned for its significant medicinal properties. Phosphorus (P), a vital nutrient for plant growth and development, has seen its levels, particularly organic P, increase in the soils of agricultural regions in southern China, presenting new challenges for nutrient management. This study aimed to uncover the molecular responses of C. wenyujin seedlings to both normal and high phosphorus (HP) conditions, shedding light on their adaptation strategies to P stress. Through transcriptome and metabolome analyses of the seedlings under normal and HP conditions, we identified 1,793 metabolites, with 195 showing differential expression. Notably, KEGG enrichment analysis highlighted 35 significantly differential accumulation metabolites (DAMs). Comparing the control group (CK) and HP treated groups (T) revealed 840 differentially expressed genes (DEGs), pinpointing the molecular divergences in response to varying P levels. Importantly, we found a potential gene, purple acid phosphatase 17 (pap17) that may cofer HP stress conditions in C. wenyujin. That elucidated the response variations of C. wenyujin seedlings to diverse P concentrations. The research suggested that C. wenyujin may adjust to varying P levels by modulating metabolites and genes linked to amino acid and phenylpropane metabolism. It highlighted the sophisticated mechanisms plants utilize to manage P stress, offering insights into their survival tactics in settings where P availability changes.
温郁金是姜科多年生草本植物，以其显著的药用特性而闻名。磷（P）是植物生长发育必需的养分，在中国南方农业区土壤中，其含量，尤其是有机磷含量有所增加，这给养分管理带来了新挑战。本研究旨在揭示温郁金幼苗在正常磷和高磷（HP）条件下的分子响应，以阐明其对磷胁迫的适应策略。通过对正常和高磷条件下幼苗的转录组和代谢组分析，我们鉴定出1793种代谢物，其中195种表现出差异表达。值得注意的是，KEGG富集分析突出了35种显著差异积累的代谢物（DAMs）。比较对照组（CK）和高磷处理组（T）发现了840个差异表达基因（DEGs），确定了对不同磷水平响应的分子差异。重要的是，我们发现了一个潜在基因——紫色酸性磷酸酶17（pap17），它可能与温郁金的高磷胁迫条件有关。这阐明了温郁金幼苗对不同磷浓度的响应变化。研究表明，温郁金可能通过调节与氨基酸和苯丙烷代谢相关的代谢物和基因来适应不同的磷水平。它突出了植物用于应对磷胁迫的复杂机制，为其在磷有效性变化环境中的生存策略提供了见解。
