Liu Zehua, Liu Jianshen, Tang Ruoxin, Zhang Zhaowan, Tian Shuangqi
Grain, Oil and Food Engineering Technology Research Center of the State Grain and Reserves Administration/Key Laboratory of Henan Province, College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China.
Food Laboratory of Zhongyuan, Henan University of Technology, Zhengzhou 450001, China.
Foods. 2024 Jun 12;13(12):1843. doi: 10.3390/foods13121843.
A whole-grain highland barley (WHB) diet has been recognized to exhibit the potential for alleviating hyperlipidemia, which is mainly characterized by lipids accumulation in the serum and liver. Previously, procyanidin B1 (PB) and coumaric acid (CA) from WHB were found to alleviate serum lipid accumulation in impaired glucose tolerance mice, while the effect on modulating the hepatic lipid metabolism remains unknown. In this study, the results showed the supplementation of PB and CA activated the expression of peroxisome proliferator-activated receptor α (PPARα) and the target genes of cholesterol 7-α hydroxylase () and carnitine palmitoyl transferase I () in the liver cells of high-fat-diet (HFD)-induced diabetic C57BL/6J mice, resulting in decreases in the serum total cholesterol (TC), triglyceride (TG), and low-density lipoprotein (LDL-C) contents, and an increase in the high-density lipoprotein (HDL-C) content. High-throughput sequencing of 16S rRNA indicated that supplementation with PB and CA ameliorated the gut microbiota dysbiosis, which was associated with a reduction in the relative abundance of and an increase in the relative abundance of , and . Spearman's correlation analysis revealed that these genera were closely related to obesity-related indices. In summary, the activation of PPARα expression by PB and CA from WHB was important for the alleviation of hyperlipidemia and the structural adjustment of the gut microbiota.
全谷物青稞（WHB）饮食已被认为具有缓解高脂血症的潜力，高脂血症主要特征是血清和肝脏中脂质积累。此前，发现来自WHB的原花青素B1（PB）和香豆酸（CA）可缓解糖耐量受损小鼠的血清脂质积累，但其对调节肝脏脂质代谢的影响尚不清楚。在本研究中，结果表明补充PB和CA可激活高脂饮食（HFD）诱导的糖尿病C57BL/6J小鼠肝细胞中过氧化物酶体增殖物激活受体α（PPARα）以及胆固醇7-α羟化酶（）和肉碱棕榈酰转移酶I（）靶基因的表达，导致血清总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白（LDL-C）含量降低，高密度脂蛋白（HDL-C）含量增加。16S rRNA高通量测序表明，补充PB和CA可改善肠道微生物群失调，这与 相对丰度降低以及 、 和 相对丰度增加有关。Spearman相关性分析显示，这些菌属与肥胖相关指标密切相关。总之，来自WHB的PB和CA激活PPARα表达对于缓解高脂血症和肠道微生物群结构调整很重要。
