Borsato Débora M, Prudente Arthur S, Döll-Boscardin Patrícia M, Borsato Aurélio V, Luz Cynthia F P, Maia Beatriz H L N S, Cabrini Daniela A, Otuki Michel F, Miguel Marilis D, Farago Paulo V, Miguel Obdulio G
1 Department of Pharmacy, Federal University of Paraná , Curitiba, Brazil .
J Med Food. 2014 Jul;17(7):817-25. doi: 10.1089/jmf.2013.0024. Epub 2014 Mar 20.
Melipona marginata is an endangered species of stingless bee from Brazil that produces honey with particular physicochemical features and a remarkable exotic flavor. To the best of our knowledge, this is the first report devoted to exploring the medicinal potential of this honey. Thus, the aim of this paper was to investigate the potential anti-inflammatory activity of honey extract from M. marginata on skin inflammation. The honey sample was classified as a monofloral honey of Mimosa scabrella. The presence of 11 phenolic compounds as kaempferol and caffeic acid was detected using the high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-UV-ESI-MS) method. The anti-inflammatory activity was measured using a 12-O-tetradecanoylphorbol-13-acetate-induced ear edema model of inflammation in mice. The topical application of the M. marginata honey extract (1.0 mg/ear) was able to reduce ear edema with an inhibitory effect of 54 ± 5%. This extract decreased the myeloperoxidase activity in 75 ± 3%, which suggests a lower leucocyte infiltration that was confirmed by histological analysis. This extract also provided a reduction of 55 ± 14% in the production of reactive oxygen species. This anti-inflammatory activity could be due to a synergic effect of the phenolic compounds identified in the honey sample. Taken together, these results open up new possibilities for the use of M. marginata honey extract in skin disorders.
黄纹无刺蜂是一种来自巴西的濒危无刺蜂，其所产蜂蜜具有独特的理化特性和显著的异国风味。据我们所知，这是首篇致力于探索这种蜂蜜药用潜力的报告。因此，本文旨在研究黄纹无刺蜂蜜提取物对皮肤炎症的潜在抗炎活性。该蜂蜜样本被归类为含羞草单花蜂蜜。采用高效液相色谱 - 电喷雾电离 - 串联质谱法（HPLC - UV - ESI - MS）检测到11种酚类化合物的存在，如槲皮素和咖啡酸。使用12 - O - 十四酰佛波醇 - 13 - 乙酸酯诱导的小鼠耳部炎症水肿模型来测定抗炎活性。局部应用黄纹无刺蜂蜜提取物（1.0毫克/耳）能够减轻耳部水肿，抑制率为54±5%。该提取物使髓过氧化物酶活性降低了75±3%，这表明白细胞浸润减少，组织学分析证实了这一点。该提取物还使活性氧的产生减少了55±14%。这种抗炎活性可能归因于蜂蜜样本中鉴定出的酚类化合物的协同作用。综上所述，这些结果为黄纹无刺蜂蜜提取物在皮肤疾病中的应用开辟了新的可能性。
