Dayyoub Ghaffar, Al-Tayyan Mouhammad, Alsayed Tolibah Yasser, Achour Hassan
Department of Endodontics, Faculty of dentistry, Damascus University, P.O. Box 3062, Damascus, Syria.
Department of Pediatric Dentistry, Faculty of dentistry, Damascus University, P.O. Box 3062, Damascus, Syria.
Sci Rep. 2025 Feb 21;15(1):6319. doi: 10.1038/s41598-025-90841-3.
To assess the effects of 5.25% sodium hypochlorite (NaOCl), 17% ethylenediaminetetraacetic acid (EDTA), and 2% chlorhexidine (CHX), individually and in combination, as part of an irrigation protocol on furcal perforations treated with ProRoot MTA, using the dye extraction (DE) method for evaluation. Sixty intact mandibular molars were used. Perforations were created with a 1.2 mm diameter at the FA of the molars and sealed the perforations with MTA. Then the samples were divided randomly after to six groups according to the irrigant used: Group 1 (n = 10), 5.25% NaOCl; Group 2 (n = 10), 17% EDTA; Group 3 (n = 10), 2% CHX, Group 4 (n = 10), saline serum (control group); Group 5 (n = 10), (5.25% NaOCl,17% EDTA, 5.25% NaOCl) protocol replaced with saline serum between each irrigant; and Group 6 (n = 10), (5.25% NaOCl, 17% EDTA, 2% CHX). Specimens were immersed for 24 h in 2% methylene blue. Subsequently, a DE test was performed. Data analysis employed the One-Way ANOVA and LSD tests. The significance level was set at 0.05. DE values between groups revealed significant differences in this study (p < 0.01). Control group DE values were less than NaOCl (p < 0.05), EDTA (p < 0.01), CHX (p < 0.01), protocol (NaOCl - EDTA - NaOCl) (p < 0.01), and protocol (NaOCl - EDTA - CHX) (p < 0.01) groups, proposing the order: Control group < NaOCl < EDTA < CHX≤(NaOCl - EDTA - NaOCl) protocol≤(NaOCl - EDTA - CHX) protocol. Deterioration of the MTA sealing ability was noted when contacted with 5.25% NaOCl, 17% EDTA, 2% CHX, and both protocols.
为了评估5.25%次氯酸钠（NaOCl）、17%乙二胺四乙酸（EDTA）和2%氯己定（CHX）单独及联合使用作为冲洗方案的一部分，对采用ProRoot MTA治疗的根分叉穿孔的影响，使用染料提取（DE）法进行评估。使用了60颗完整的下颌磨牙。在磨牙的远中根分叉处用直径1.2毫米的钻头制造穿孔，并用MTA封闭穿孔。然后根据使用的冲洗剂将样本随机分为六组：第1组（n = 10），5.25% NaOCl；第2组（n = 10），17% EDTA；第3组（n = 10），2% CHX；第4组（n = 10），生理盐水血清（对照组）；第5组（n = 10），（5.25% NaOCl、17% EDTA、5.25% NaOCl）方案，每种冲洗剂之间用生理盐水血清替换；第6组（n = 10），（5.25% NaOCl、17% EDTA、2% CHX）。将样本在2%亚甲蓝中浸泡24小时。随后，进行DE试验。数据分析采用单因素方差分析和LSD检验。显著性水平设定为0.05。组间DE值在本研究中显示出显著差异（p < 0.01）。对照组DE值低于NaOCl组（p < 0.05）、EDTA组（p < 0.01）、CHX组（p < 0.01）、方案（NaOCl - EDTA - NaOCl）组（p < 0.01）和方案（NaOCl - EDTA - CHX）组（p < 0.01），得出顺序为：对照组 < NaOCl < EDTA < CHX≤（NaOCl - EDTA - NaOCl）方案≤（NaOCl - EDTA - CHX）方案。当与5.25% NaOCl、17% EDTA、2% CHX以及两种方案接触时，观察到MTA封闭能力下降。
