Hsu Peihan, Kamijyo Yuriko, Koike Emiri, Ichikawa Saki, Zheng Yifeng, Ohno Tomohiro, Katayama Shigeru
Department of Agriculture, Graduate School of Science and Technology, Shinshu University, Nagano, Japan.
Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Nagano, Japan.
Front Nutr. 2025 Feb 10;12:1486572. doi: 10.3389/fnut.2025.1486572. eCollection 2025.
Plant-derived exosome-like nanovesicles (ELNs) are critical mediators of cross-kingdom communication, modulating gene expression in animal cells despite their plant origin. In this study, we investigated the effects of glucoraphanin-enriched kale (GEK)-derived ELNs (GELNs) on collagen production in normal human dermal fibroblasts NB1RGB. The ELNs isolated from GEK juice powder had particle sizes similar to those of typical exosomes. GELNs increased type I collagen expression in NB1RGB cells significantly. Microarray analysis demonstrated that GELN-derived total RNA upregulated the expression of genes related to extracellular matrix formation, including those involved in collagen synthesis. Further investigation revealed that microRNA-enriched fraction of GELNs promoted collagen production by inhibiting the expression of Smad7. These findings suggest that GELNs and their microRNA content enhance collagen production through the downregulation of Smad7.
植物来源的类外泌体纳米囊泡（ELNs）是跨界通讯的关键介质，尽管其来源于植物，但却能调节动物细胞中的基因表达。在本研究中，我们调查了富含萝卜硫苷的羽衣甘蓝（GEK）来源的ELNs（GELNs）对正常人皮肤成纤维细胞NB1RGB中胶原蛋白产生的影响。从GEK汁粉中分离出的ELNs的粒径与典型外泌体相似。GELNs显著增加了NB1RGB细胞中I型胶原蛋白的表达。微阵列分析表明，GELN来源的总RNA上调了与细胞外基质形成相关的基因的表达，包括参与胶原蛋白合成的基因。进一步研究发现，GELNs中富含微小RNA的部分通过抑制Smad7的表达促进了胶原蛋白的产生。这些发现表明，GELNs及其微小RNA含量通过下调Smad7增强了胶原蛋白的产生。
