Iurilli Martin, Porrelli Davide, Turco Gianluca, Lagatolla Cristina, Camurri Piloni Alvise, Medagli Barbara, Nicolin Vanessa, Papa Giovanni
Plastic and Reconstructive Surgery Unit, Cattinara Hospital, Strada di Fiume, 447, 34149 Trieste, Italy.
Clinical Department of Medical, Surgical and Health Sciences, University of Trieste, Strada di Fiume, 447, 34149 Trieste, Italy.
Membranes (Basel). 2025 Feb 1;15(2):39. doi: 10.3390/membranes15020039.
Complex wounds pose a significant healthcare challenge due to their susceptibility to infections and delayed healing. This study focuses on developing electrospun polycaprolactone (PCL) nanofiber membranes coated with Type I collagen derived from bovine skin and functionalized with silver nanoparticles (AgNPs) to address these issues. The collagen coating enhances biocompatibility, while AgNPs synthesized through chemical reduction with sodium citrate provide broad-spectrum antimicrobial properties. The physical properties of the membranes were characterized using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Results showed the formation of nanofibers without defects and the uniform distribution of AgNPs. A swelling test and contact angle measurements confirmed that the membranes provided an optimal environment for wound healing. In vitro biological assays with murine 3T3 fibroblasts revealed statistically significant ( ≤ 0.05) differences in cell viability among the membranes at 24 h ( = 0.0002) and 72 h ( = 0.022), demonstrating the biocompatibility of collagen-coated membranes and the minimal cytotoxicity of AgNPs. Antibacterial efficacy was evaluated against (SA), (PA), and Vancomycin-resistant (VRE), with the significant inhibition of biofilm formation observed for VRE ( = 0.006). Overall, this novel combination of collagen-coated electrospun PCL nanofibers with AgNPs offers a promising strategy for advanced wound dressings, providing antimicrobial benefits. Future in vivo studies are warranted to further validate its clinical and regenerative potential.
复杂伤口由于易感染和愈合延迟，给医疗保健带来了重大挑战。本研究聚焦于开发一种电纺聚己内酯（PCL）纳米纤维膜，该膜涂覆有源自牛皮的I型胶原蛋白，并通过银纳米颗粒（AgNP）进行功能化，以解决这些问题。胶原蛋白涂层增强了生物相容性，而通过柠檬酸钠化学还原合成的AgNP具有广谱抗菌性能。使用扫描电子显微镜（SEM）和原子力显微镜（AFM）对膜的物理性质进行了表征。结果显示形成了无缺陷的纳米纤维且AgNP分布均匀。肿胀试验和接触角测量证实，这些膜为伤口愈合提供了最佳环境。对小鼠3T3成纤维细胞进行的体外生物学测定显示，在24小时（P = 0.0002）和72小时（P = 0.022）时，各膜之间的细胞活力存在统计学显著差异（P≤0.05），这证明了胶原蛋白涂层膜的生物相容性以及AgNP的最小细胞毒性。评估其对金黄色葡萄球菌（SA）、铜绿假单胞菌（PA）和耐万古霉素肠球菌（VRE）的抗菌效果，观察到对VRE生物膜形成有显著抑制作用（P = 0.006）。总体而言，这种胶原蛋白涂层的电纺PCL纳米纤维与AgNP的新型组合为先进伤口敷料提供了一种有前景的策略，具有抗菌益处。未来有必要进行体内研究以进一步验证其临床和再生潜力。
