Gensler H L, Lindberg R E, Pinnas J L, Jones J F
Leuk Res. 1985;9(4):471-9. doi: 10.1016/0145-2126(85)90006-2.
The capacity of human thymocytes and of differentiated lymphocytes circulating in peripheral blood to perform unscheduled DNA synthesis (a measure of nucleotide excision repair) after UV irradiation was measured by radioautographic analysis. Only 4% of immature T lymphocytes, but 68% of circulating lymphocytes exhibited unscheduled DNA synthesis. When UV sensitivity of peripheral blood lymphocytes and thymocytes from the same donor were compared, the thymocytes, in each case, were significantly more UV sensitive than were the circulating lymphocytes. Peripheral blood lymphocytes from subjects undergoing halothane and morphine anesthesia during surgery showed 56% less excision repair capacity than those from unanesthetized donors. The difference occurred in the number of cells capable of repair rather than in the extent of repair synthesis per cell. Ultraviolet-induced unscheduled DNA synthesis occurred in only 3% of the thymocytes removed from rats killed by cervical dislocation. Therefore, the deficiency of excision repair was observed in rat thymocytes which had not been affected by anesthesia or surgical trauma. Since the thymus contains more than 90% immature T-cells, our results indicate that immature T-cells are deficient in nucleotide excision repair whereas the majority of mature peripheral blood lymphocytes exhibit such repair.
通过放射自显影分析，测定了人类胸腺细胞以及外周血中循环的分化淋巴细胞在紫外线照射后进行非预定DNA合成（核苷酸切除修复的一种衡量指标）的能力。只有4%的未成熟T淋巴细胞表现出非预定DNA合成，而循环淋巴细胞中有68%表现出非预定DNA合成。当比较来自同一供体的外周血淋巴细胞和胸腺细胞的紫外线敏感性时，在每种情况下，胸腺细胞对紫外线的敏感性都显著高于循环淋巴细胞。接受氟烷和吗啡麻醉的手术患者的外周血淋巴细胞，其切除修复能力比未麻醉供体的外周血淋巴细胞低56%。这种差异在于能够进行修复的细胞数量，而非每个细胞的修复合成程度。从颈椎脱臼处死的大鼠体内取出的胸腺细胞中，只有3%发生了紫外线诱导的非预定DNA合成。因此，在未受麻醉或手术创伤影响的大鼠胸腺细胞中观察到了切除修复缺陷。由于胸腺中超过90%是未成熟T细胞，我们的结果表明，未成熟T细胞缺乏核苷酸切除修复能力，而大多数成熟外周血淋巴细胞具有这种修复能力。
