Thomas Emily M, Smith Emma M, Papke Erin, Shlafstein Maximillian D, Grant-Beurmann Silvia, Coelho Lívia C, Trott Alison C, Häse Claudia C, Oline David K, Videau Patrick, Saw Jimmy H, Strangman Wendy K, Ushijima Blake
Department of Biology and Marine Biology, University of North Carolina Wilmington, Wilmington, North Carolina, USA.
Department of Chemistry and Biochemistry, University of North Carolina Wilmington, Wilmington, North Carolina, USA.
Int J Syst Evol Microbiol. 2025 Feb;75(2). doi: 10.1099/ijsem.0.006681.
Strains P94, B95 and R96 were isolated from apparently healthy fragments of the coral , which were resistant to infection, from the reef surrounding Moku o Lo'e in Kāne'ohe Bay, O'ahu, Hawai'i, USA, and were taxonomically evaluated using a polyphasic approach. Phylogenetic and phylogenomic analyses placed strains P94, B95 and R96 within the genus and most closely related to and . Following genome sequencing of strains P94, B95 and R96, the average nt identity and DNA-DNA hybridization comparisons with closely related strains resulted in values that fell below species-level cutoffs. Based on a polyphasic characterization and differences in genomic and taxonomic data, strains P94, B95 and R96 represent novel species, for which the names sp. nov., sp. nov. and sp. nov. are proposed. The type strains are R96 (=DSM 114998=LMG 32870), P94 (=DSM 114996=LMG 32871) and B95 (=DSM 114997=LMG 32872), respectively.
菌株P94、B95和R96是从美国夏威夷欧胡岛卡内奥赫湾莫库奥洛埃岛周围珊瑚礁中表面健康且具有抗感染能力的珊瑚碎片中分离出来的，并采用多相分类法对其进行了分类学评估。系统发育和系统基因组分析将菌株P94、B95和R96置于该属内，且与[具体菌种1]和[具体菌种2]关系最为密切。对菌株P94、B95和R96进行基因组测序后，与密切相关菌株的平均核苷酸同一性和DNA-DNA杂交比较结果低于物种水平的阈值。基于多相分类特征以及基因组和分类学数据的差异，菌株P94、B95和R96代表新物种，分别命名为[新物种名1] sp. nov.、[新物种名2] sp. nov.和[新物种名3] sp. nov.。模式菌株分别为R96（=DSM 114998=LMG 32870）、P94（=DSM 114996=LMG 32871）和B95（=DSM 114997=LMG 32872）。
