Beurmann Silvia, Ushijima Blake, Svoboda Christina M, Videau Patrick, Smith Ashley M, Donachie Stuart P, Aeby Greta S, Callahan Sean M
Present address: Institute for GenomeSciences, School of Medicine, University of Maryland, Baltimore, MD 21201, USA.
Hawai'i Institute of Marine Biology, 46-007 Lilipuna Road, Kāne'ohe, HI 96744, USA.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2683-2688. doi: 10.1099/ijsem.0.001995. Epub 2017 Aug 18.
A Gram-stain-negative, motile, rod-shaped bacterium designated OCN003T was cultivated from mucus taken from a diseased colony of the coral Montipora capitata in Kāne'ohe Bay, O'ahu, Hawai'i. Colonies of OCN003T were pale yellow, 1-3 mm in diameter, convex, smooth and entire. The strain was heterotrophic, strictly aerobic and strictly halophilic. Cells of OCN003T produced buds on peritrichous prosthecae. Growth occurred within the pH range of 5.5 to 10, and the temperature range of 14 to 39 °C. Major fatty acids were 16 : 1ω7c, 16 : 0, 18 : 1ω7c, 17 : 1ω8c, 12 : 0 3-OH and 17 : 0. Phylogenetic analysis of 1399 nucleotides of the 16S rRNA gene nucleotide sequence and a multi-locus sequence analysis of three genes placed OCN003T in the genus Pseudoalteromonas and indicated that the nearest relatives described are Pseudoalteromonas spongiae, P. luteoviolacea, P. ruthenica and P. phenolica(97-99 % sequence identity). The DNA G+C content of the strain's genome was 40.0 mol%. Based on in silico DNA-DNA hybridization and phenotypic differences from related type strains, we propose that OCN003T represents the type strain of a novel species in the genus Pseudoalteromonas, proposed as Pseudoalteromonas piratica sp. nov. OCN003T (=CCOS1042T=CIP 111189T). An emended description of the genus Pseudoalteromonas is presented.
从夏威夷欧胡岛卡内奥赫湾患病的头状蔷薇珊瑚菌落的黏液中培养出了一株革兰氏染色阴性、具运动性、杆状的细菌，命名为OCN003T。OCN003T的菌落呈浅黄色，直径1-3毫米，凸起、光滑且边缘整齐。该菌株为异养型，严格需氧且严格嗜盐。OCN003T的细胞在周生菌柄上产生芽体。在pH值5.5至10以及温度14至39°C范围内生长。主要脂肪酸为16:1ω7c、16:0、18:1ω7c、17:1ω8c、12:0 3-OH和17:0。对16S rRNA基因核苷酸序列的1399个核苷酸进行系统发育分析以及对三个基因进行多位点序列分析，将OCN003T置于假交替单胞菌属中，表明其最接近的已知亲缘种是海绵假交替单胞菌、黄紫假交替单胞菌、鲁氏假交替单胞菌和酚假交替单胞菌（序列同一性为97-99%）。该菌株基因组的DNA G+C含量为40.0摩尔%。基于电子DNA-DNA杂交以及与相关模式菌株的表型差异，我们提议OCN003T代表假交替单胞菌属中一个新物种的模式菌株，并将其命名为海盗假交替单胞菌（Pseudoalteromonas piratica）新种。OCN003T（=CCOS1042T=CIP 111189T）。本文还给出了假交替单胞菌属的修订描述。
