Tipu Jahid Hasan, Miah Rijon, Islam Obaidul, Rahman Md Mukidur, Talukdar Lucky, Miah Rubel, Hussain Md Safwan, Islam Md Ashraful, Ahsan Md Irtija, Raquib Ahsan, Noor Monira
Centre for International Health, Department of Global Public Health and Primary Care, Faculty of Medicine, University of Bergen, Bergen, Norway.
Department of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh.
Vet Med Sci. 2025 Mar;11(2):e70227. doi: 10.1002/vms3.70227.
Mycoplasma gallisepticum (MG) poses a significant threat to Bangladesh's poultry industry, causing substantial economic losses every year. This study aimed to determine the prevalence of MG infection in turkeys using serum plate agglutination (SPA), enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) in Sylhet, Bangladesh from December 2019 to November 2020. In addition, we evaluated the diagnostic accuracy of these tests and identified potential risk factors associated with MG infection. A total of 250 blood samples and 250 tracheal swabs were collected from suspected turkeys across 25 farms from three sub-districts of Sylhet namely Sylhet Sadar, Golapganj and Beanibazar. Blood samples were tested with SPA and ELISA, while tracheal swabs were analysed by PCR targeting the 16S rRNA gene of MG. The overall prevalence of MG was 35.2%, 29.2% and 25.6% for SPA, ELISA and PCR respectively. Higher infection rates were observed in turkeys aged 0-4 months (SPA 57.1%, ELISA 52%, PCR 42.8%), during winter (SPA 43.1%, ELISA 37.8%, PCR 30%) and among female turkeys (SPA 54.5%, ELISA 49.5%, PCR 45.5%). Geographically, the Beanibazar had the highest prevalence (SPA 54.2%, ELISA 48.6%, PCR 41.4%), compared to the Sylhet Sadar and Golapganj sub-districts. Both SPA and ELISA tests showed 100% sensitivity, with specificity of 87.1% and 95.2%, respectively using PCR as a gold standard. Overall, these findings provide valuable insights for developing effective control measures for MG infections in the poultry industry of Bangladesh.
鸡毒支原体（MG）对孟加拉国的家禽业构成重大威胁，每年造成巨大经济损失。本研究旨在通过血清平板凝集试验（SPA）、酶联免疫吸附测定（ELISA）和聚合酶链反应（PCR），确定2019年12月至2020年11月期间孟加拉国锡尔赫特地区火鸡中MG感染的流行情况。此外，我们评估了这些检测方法的诊断准确性，并确定了与MG感染相关的潜在风险因素。从锡尔赫特三个分区（锡尔赫特萨达尔、戈拉普甘杰和贝尼巴扎尔）的25个农场的疑似火鸡中，共采集了250份血液样本和250份气管拭子。血液样本用SPA和ELISA进行检测，而气管拭子则通过针对MG的16S rRNA基因进行PCR分析。SPA、ELISA和PCR检测出的MG总体流行率分别为35.2%、29.2%和25.6%。在0至4个月龄的火鸡中（SPA为57.1%，ELISA为52%，PCR为42.8%）、冬季（SPA为43.1%，ELISA为37.8%，PCR为30%）以及雌性火鸡中（SPA为54.5%，ELISA为49.5%，PCR为45.5%）观察到较高的感染率。在地理分布上，与锡尔赫特萨达尔和戈拉普甘杰分区相比，贝尼巴扎尔的流行率最高（SPA为54.2%，ELISA为48.6%，PCR为41.4%）。以PCR作为金标准，SPA和ELISA检测的灵敏度均为100%，特异性分别为87.1%和95.2%。总体而言，这些研究结果为孟加拉国家禽业制定有效的MG感染控制措施提供了有价值的见解。
