Amitrano Alice, Choudhury Debanik, Konstantopoulos Konstantinos
Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA; Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA; Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore, MD 21218, USA; Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA; Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Baltimore, MD 21231, USA.
Curr Opin Cell Biol. 2025 Jun;94:102487. doi: 10.1016/j.ceb.2025.102487. Epub 2025 Feb 24.
Cell migration through confined spaces is a critical process influenced by the complex three-dimensional (3D) architecture of the local microenvironment and the surrounding extracellular matrix (ECM). Cells in vivo experience diverse fluidic signals, such as extracellular fluid viscosity, hydraulic resistance, and shear forces, as well as solid cues, like ECM stiffness and viscoelasticity. These fluidic and solid stressors activate mechanotransduction processes and regulate cell migration. They also drive metabolic reprogramming, dynamically altering glycolysis and oxidative phosphorylation to meet the cell's energy demands in different microenvironments. This review discusses recent advances on the mechanisms of cell migration in confinement and how confinement-induced cellular behavior leads to metabolic reprogramming.
细胞通过狭窄空间的迁移是一个关键过程，受到局部微环境复杂的三维（3D）结构和周围细胞外基质（ECM）的影响。体内的细胞会经历多种流体信号，如细胞外液粘度、水力阻力和剪切力，以及固体信号，如ECM硬度和粘弹性。这些流体和固体应激源激活机械转导过程并调节细胞迁移。它们还驱动代谢重编程，动态改变糖酵解和氧化磷酸化，以满足细胞在不同微环境中的能量需求。本综述讨论了细胞在受限环境中迁移机制的最新进展，以及受限诱导的细胞行为如何导致代谢重编程。
