Alexander S, Shinnick T M
Mol Cell Biol. 1985 May;5(5):984-90. doi: 10.1128/mcb.5.5.984-990.1985.
Dictyostelium discoideum strains that carry the dis mutations fail to express the family of developmentally regulated discoidin lectin genes during morphogenesis. We show here that this absence of discoidin lectin expression is due to the failure to transcribe the discoidin genes. Furthermore, the dis mutations appear to affect only discoidin expression and not the expression of other proteins during development, as assessed by a two-dimensional gel analysis of pulse-labeled proteins and by the accumulation of developmentally regulated enzymes. The dis mutations appear to define trans-acting regulatory loci, the products of which act at the transcriptional level to control specifically the developmental expression of the discoidin gene family.
携带dis突变的盘基网柄菌菌株在形态发生过程中无法表达发育调控的盘基网柄菌凝集素基因家族。我们在此表明，盘基网柄菌凝集素表达的缺失是由于无法转录盘基网柄菌基因。此外，通过对脉冲标记蛋白质的二维凝胶分析以及发育调控酶的积累评估，dis突变似乎仅影响盘基网柄菌的表达，而不影响发育过程中其他蛋白质的表达。dis突变似乎定义了反式作用调控位点，其产物在转录水平起作用，以特异性控制盘基网柄菌基因家族的发育表达。
