Rowekamp W, Poole S, Firtel R A
Cell. 1980 Jun;20(2):495-505. doi: 10.1016/0092-8674(80)90636-4.
Using recombinant DNA technology, we have isolated chimeric plasmids carrying genomic or cDNA sequences complementary to mRNA encoding the developmentally regulated carbohydrate-binding protein Discoidin-I. The protein is encoded by a 4-5 member multigene family. Mapping of the genomic sequences by DNA blot hybridization suggests that several of the genes are closely linked. RNA excess hybridization kinetics indicate that Discoidin-I mRNA is found in less than one copy per gene per cell during vegetative growth, and increases 500-1000 fold during the first 6 hr of development. The Discoidin-I mRNA concentration decreases during later stages of development. mRNA complementary to the genes shows two bands of -1.0-1.1 kb on acrylamide gels, indicating heterogeneity either in the genes or in mRNA processing.
利用重组DNA技术，我们分离出了嵌合质粒，这些质粒携带与编码发育调控型碳水化合物结合蛋白盘状蛋白I的mRNA互补的基因组或cDNA序列。该蛋白由一个由4 - 5个成员组成的多基因家族编码。通过DNA印迹杂交对基因组序列进行定位表明，其中几个基因紧密连锁。RNA过量杂交动力学表明，在营养生长期间，每个细胞中每个基因的盘状蛋白I mRNA拷贝数少于一个，而在发育的最初6小时内增加500 - 1000倍。盘状蛋白I mRNA浓度在发育后期降低。与这些基因互补的mRNA在丙烯酰胺凝胶上显示出两条-1.0 - 1.1 kb的条带，表明基因或mRNA加工过程中存在异质性。
