Devine J M, Williams J G
Nucleic Acids Res. 1982 Feb 25;10(4):1231-41. doi: 10.1093/nar/10.4.1231.
The discoidin I genes of Dictyostelium discoideum encode a family of three closely related developmentally regulated lectins which may play a role in cell cohesion. We have isolated a genomic clone containing the 5' half of a discoidin I gene with 12kb of flanking sequence and we have identified the discoidin I polypeptide encoded by this gene. We have determined part of the nucleotide sequence of the cloned segment and have hence determined the N-terminal amino acid sequence of the encoded polypeptide. The nucleotide sequence upstream of the AUG initiation codon was unusually AT rich, with 94% AT base pairs in the 171 nucleotides for which sequence was determined. However, having determined the start point of transcription ('cap' site) two striking features of similarity with other eukaryotic structural genes became apparent. The sequence TATAAA was present 33 nucleotides upstream from the 'cap' site and the sequence CCAAT was present a further 20 nucleotides upstream.
盘基网柄菌（Dictyostelium discoideum）的盘状I基因编码了一组由三个密切相关的、受发育调控的凝集素组成的家族，这些凝集素可能在细胞黏附中发挥作用。我们分离出了一个基因组克隆，它包含一个盘状I基因5'端的一半以及12kb的侧翼序列，并且我们已经鉴定出了由该基因编码的盘状I多肽。我们确定了克隆片段的部分核苷酸序列，从而确定了所编码多肽的N端氨基酸序列。AUG起始密码子上游的核苷酸序列富含AT，在已确定序列的171个核苷酸中，AT碱基对占94%。然而，在确定转录起始点（“帽”位点）后，与其他真核结构基因相似的两个显著特征变得明显。序列TATAAA出现在“帽”位点上游33个核苷酸处，序列CCAAT则出现在再上游20个核苷酸处。
