Gao X, Gaffney B L, Senior M, Riddle R R, Jones R A
Nucleic Acids Res. 1985 Jan 25;13(2):573-84. doi: 10.1093/nar/13.2.573.
Thymine residues in an oligodeoxyribonucleotide are subject to methylation at N3 by the internucleotide methyl phosphotriester linkages. This alkylation occurs most rapidly in the presence of a strong base such as DBU, but also takes place, at a much slower rate, during oligonucleotide synthesis.
寡脱氧核糖核苷酸中的胸腺嘧啶残基会通过核苷酸间甲基磷酸三酯键在N3位发生甲基化。这种烷基化在强碱(如DBU)存在的情况下发生得最快,但在寡核苷酸合成过程中也会以慢得多的速度发生。