Efimov V A, Reverdatto S V, Chakhmakhcheva O G
Nucleic Acids Res. 1982 Nov 11;10(21):6675-94. doi: 10.1093/nar/10.21.6675.
A rapid and convenient method for the synthesis of deoxyribooligonucleotides has been developed using the phosphotriester approach. The advantage of this methodology for work in solution was successfully demonstrated in synthesis of a number of DNA fragments up to 32-long. Adaptation of the presented method to solid-phase synthesis allows a pentadecamer to be assembled in 4-5 hours using dinucleotides as coupling units.
已开发出一种使用磷酸三酯法快速便捷地合成脱氧核糖寡核苷酸的方法。该方法在溶液中进行合成时的优势已在多个长达32个碱基的DNA片段合成中得到成功证明。将所提出的方法应用于固相合成,以二核苷酸作为偶联单元,可在4至5小时内组装出一个十五聚体。