使用携带磷酸基团2-(1-甲基咪唑-2-基)苯基保护的全碱基保护的磷酸二酯构建块进行高效的寡脱氧核糖核苷酸合成。
Highly efficient oligodeoxyribonucleotide synthesis using fully base protected phosphodiester building blocks carrying 2-(1-methylimidazol-2-yl) phenyl protection of the phosphate.
作者信息
Sproat B S, Rider P, Beijer B
出版信息
Nucleic Acids Res. 1986 Feb 25;14(4):1811-24. doi: 10.1093/nar/14.4.1811.
Abstract
Four fully base protected phosphodiester building blocks have been synthesised and fully characterised. The phosphate protecting group used was the 2-(1-methylimidazol-2-yl)phenyl group, enabling intramolecular catalysis of the condensation step in oligodeoxyribonucleotide synthesis by the solid phase phosphotriester method. Cycle times of about 12 min could thus be achieved. Moreover, the used of extra protecting groups on deoxythymidine and 2'-deoxyguanosine resulted in much cleaner oligodeoxyribonucleotides as evidenced by ion-exchange and reversed phase h.p.l.c.
摘要
已合成并全面表征了四种完全碱基保护的磷酸二酯构建模块。所使用的磷酸酯保护基团是2-(1-甲基咪唑-2-基)苯基，它能够通过固相磷三酯法在寡脱氧核糖核苷酸合成中对缩合步骤进行分子内催化。因此可以实现约12分钟的循环时间。此外，在脱氧胸苷和2'-脱氧鸟苷上使用额外的保护基团，通过离子交换和反相高效液相色谱法证明，得到的寡脱氧核糖核苷酸纯度更高。
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