Liao Jian, Lai Zhuoxin, He Chuanmeng, Li Gaocong, Dong Zhongdian, Guo Yusong, Wang Zhongduo
Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animals of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, China.
Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Aquaculture, College of Fisheries, Guangdong Ocean University, Zhanjiang, 524088, China.
Sci Data. 2025 Feb 26;12(1):340. doi: 10.1038/s41597-025-04673-x.
The Pacific whiteleg shrimp (Penaeus vannamei, synonym Lipopenaeus vannamei) is an important aquaculture species, and its breeding improvement relies heavily on high-quality genomic resources. Here, we sequenced the genome of the Pacific whiteleg shrimp using PacBio and Hi-C technologies, resulting in a 1.7 Gb genome assembly with a Contig N50 of 57.65Kb and a Scaffold N50 of 605Kb.BUSCO analysis indicated that our genome assembly achieved a gene coverage of 84.6%, a figure considered high among crustacean reference genomes. In total, 907,237,377 bp of repetitive sequences (accounting for 50.48% of the assembled genome) and 22,923 protein-coding genes were identified. Utilizing Hi-C technology, we anchored 14,604 contigs onto 44 chromosomes, yielding a chromosome-level genome assembly with a contig N50 of 42,894,961 bp. In summary, our high-quality genome assembly provides valuable reference genomic resources for the improvement and breeding of elite cultivars of the Pacific whiteleg shrimp.
南美白对虾（凡纳滨对虾，同义词为凡纳利对虾）是一种重要的水产养殖品种，其育种改良在很大程度上依赖于高质量的基因组资源。在此，我们利用PacBio和Hi-C技术对南美白对虾的基因组进行了测序，得到了一个1.7Gb的基因组组装结果，其Contig N50为57.65Kb，Scaffold N50为605Kb。BUSCO分析表明，我们的基因组组装实现了84.6%的基因覆盖率，这一数字在甲壳类参考基因组中被认为是较高的。总共鉴定出907,237,377bp的重复序列（占组装基因组的50.48%）和22,923个蛋白质编码基因。利用Hi-C技术，我们将14,604个重叠群定位到44条染色体上，得到了一个染色体水平的基因组组装结果，其重叠群N50为42,894,961bp。总之，我们高质量的基因组组装为南美白对虾优良品种的改良和育种提供了有价值的参考基因组资源。
