Pan Qianhui, Zhang Yirong, Zhang Xiangyu, Yang Yi, Huang Kecheng, Liu Chenglan
National Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
Shenzhen Noposion Crop Science CO., Ltd, Shenzhen 518102, China.
J Hazard Mater. 2025 Jun 5;489:137756. doi: 10.1016/j.jhazmat.2025.137756. Epub 2025 Feb 25.
The widespread presence of antibiotic resistance genes (ARGs) threatens ecological security and human health. In agricultural production, the simultaneous presence of non-antibiotic substances fungicides and biochar utilized for soil remediation has unclear effects on the spread of ARGs in the soil-vegetable systems. Herein, this study conducted a pots experiment and found that biochar significantly reduced pyraclostrobin accumulation in the soil and lettuce roots. Simultaneously, the co-exposure of pyraclostrobin and biochar increased the microbial community alpha diversity and the abundance of ARGs in soil, while promoting the transfer of ARGs from soil to lettuce. Proteobacteria were identified as potential primary carriers of ARGs. Planting lettuce mitigated the effects of pyraclostrobin or/and biochar on ARGs accumulation in soil. Furthermore, MGEs and bacterial community abundance were the most important direct factors increasing ARGs in soil and lettuce. Overall, these findings evaluated the combined effects of non-antibiotic substances fungicides and soil remediation materials biochar on the generation and transmission of ARGs, providing potential strategies for controlling ARGs transfer in soil-vegetable systems.
抗生素抗性基因（ARGs）的广泛存在威胁着生态安全和人类健康。在农业生产中，用于土壤修复的非抗生素物质杀菌剂和生物炭同时存在，对ARGs在土壤-蔬菜系统中的传播影响尚不清楚。在此，本研究进行了盆栽试验，发现生物炭显著降低了土壤和生菜根中吡唑醚菌酯的积累。同时，吡唑醚菌酯和生物炭共同暴露增加了土壤中微生物群落的α多样性和ARGs的丰度，同时促进了ARGs从土壤向生菜的转移。变形菌门被确定为ARGs的潜在主要载体。种植生菜减轻了吡唑醚菌酯或/和生物炭对土壤中ARGs积累的影响。此外，移动遗传元件（MGEs）和细菌群落丰度是增加土壤和生菜中ARGs的最重要直接因素。总体而言，这些发现评估了非抗生素物质杀菌剂和土壤修复材料生物炭对ARGs产生和传播的综合影响，为控制ARGs在土壤-蔬菜系统中的转移提供了潜在策略。
