Lechermann B, Jelkmann W
Respir Physiol. 1985 Apr;60(1):1-8. doi: 10.1016/0034-5687(85)90035-0.
Effects of an acute increase in blood O2 affinity on erythropoietin production were studied in normoxic and hypoxic male rats. Blood O2 affinity was increased by exchange-transfusion with blood from sodium cyanate treated rats. P50 was lowered to 27.6 torr (pH 7.4, PCO2 = 40 torr, 37 degrees C) in the recipients compared to 41.8 torr in control rats exchange transfused with normal blood. Hypoxia was induced by exposure to simulated high altitude (4750 or 7000 m) for 16 h. Erythropoietin was determined by in vivo bioassay. In rats with normal blood O2 affinity, plasma erythropoietin was undetectable at 300 m, 0.26 +/- 0.10 U/ml at 4750 m (mean +/- SEM; n = 7), and 3.52 +/- 0.58 U/ml at 7000 m (n = 10). Plasma erythropoietin titers were significantly enhanced in rats with high blood O2 affinity at 300 m (0.05 +/- 0.01 U/ml; n = 4) and moderately increased at 4750 m (0.57 +/- 0.12 U/ml; n = 7), but unchanged at 7000 m (3.88 +/- 0.74 U/ml; n = 10). These results indicate that a high blood O2 affinity reduces the O2 delivery to the cells controlling erythropoietin production in normoxia and moderate hypoxia. However, this is offset at severe hypoxia most likely by an improved O2 loading to the blood.
在常氧和低氧雄性大鼠中研究了血液氧亲和力急性增加对促红细胞生成素产生的影响。通过用氰酸钠处理的大鼠的血液进行交换输血来增加血液氧亲和力。与用正常血液进行交换输血的对照大鼠的41.8托相比，接受者的P50降低至27.6托（pH 7.4，PCO2 = 40托，37摄氏度）。通过暴露于模拟高海拔（4750或7000米）16小时诱导低氧。促红细胞生成素通过体内生物测定法测定。在血液氧亲和力正常的大鼠中，在300米处血浆促红细胞生成素不可检测，在4750米处为0.26±0.10 U/ml（平均值±标准误；n = 7），在7000米处为3.52±0.58 U/ml（n = 10）。在血液氧亲和力高的大鼠中，血浆促红细胞生成素滴度在300米处显著升高（0.05±0.01 U/ml；n = 4），在4750米处中度升高（0.57±0.12 U/ml；n = 7），但在7000米处无变化（3.88±0.74 U/ml；n = 10）。这些结果表明，高血液氧亲和力在常氧和中度低氧状态下会减少向控制促红细胞生成素产生的细胞的氧输送。然而，在严重低氧时，这很可能被血液中改善的氧装载所抵消。
