Gupta Aporva, Park Gyeongmin, Park So-Yoon, Kim Haneul, Rha Eugene, Lee Dae-Hee, Lee Seung-Goo, Kim Haseong
Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology, Daejeon 34113, Republic of Korea.
J Microbiol Biotechnol. 2025 Feb 25;35:e2412046. doi: 10.4014/jmb.2412.12046.
Synthetic biology has garnered significant global interest owing to its diverse applications in bio-based production, biosensing, living therapeutics, and drug delivery. This heightened interest has increased the demand for novel protein synthesis methods and genome-scale assemblies. However, gene assembly from oligomers presents several challenges, including the risk of incorrect assembly between oligomers, self-annealing, and the potential for insertions or deletions resulting from misannealed oligomers. Dsembler (DNA Assembly Designer) is a web-based software application designed to assemble long DNA sequences. It provides a set of oligomers with optimal melting temperatures and GC overlap, which facilitates a commercially available oligomer pool service. By enhancing the accuracy of oligomer design, Dsembler has addressed critical challenges in synthetic biology and supported advancements in genetic engineering and molecular biology.
合成生物学因其在生物基生产、生物传感、活体治疗和药物递送等方面的多样应用而在全球引起了广泛关注。这种日益增长的兴趣增加了对新型蛋白质合成方法和基因组规模组装的需求。然而，从寡聚物进行基因组装存在若干挑战，包括寡聚物之间错误组装的风险、自退火以及错配寡聚物导致插入或缺失的可能性。Dsembler（DNA组装设计器）是一款基于网络的软件应用程序，旨在组装长DNA序列。它提供了一组具有最佳解链温度和GC重叠的寡聚物，这有助于开展商用寡聚物文库服务。通过提高寡聚物设计的准确性，Dsembler解决了合成生物学中的关键挑战，并支持了基因工程和分子生物学的进展。
