Hughes Randall A, Ellington Andrew D
Applied Research Laboratories, The University of Texas at Austin, Austin, Texas 78758.
Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712.
Cold Spring Harb Perspect Biol. 2017 Jan 3;9(1):a023812. doi: 10.1101/cshperspect.a023812.
The chemical synthesis of DNA oligonucleotides and their assembly into synthons, genes, circuits, and even entire genomes by gene synthesis methods has become an enabling technology for modern molecular biology and enables the design, build, test, learn, and repeat cycle underpinning innovations in synthetic biology. In this perspective, we briefly review the techniques and technologies that enable the synthesis of DNA oligonucleotides and their assembly into larger DNA constructs with a focus on recent advancements that have sought to reduce synthesis cost and increase sequence fidelity. The development of lower-cost methods to produce high-quality synthetic DNA will allow for the exploration of larger biological hypotheses by lowering the cost of use and help to close the DNA read-write cost gap.
DNA寡核苷酸的化学合成以及通过基因合成方法将它们组装成合成子、基因、电路乃至整个基因组，已成为现代分子生物学的一项使能技术，并促成了合成生物学创新所依赖的设计、构建、测试、学习和重复循环。从这个角度出发，我们简要回顾一下使DNA寡核苷酸得以合成并将其组装成更大DNA构建体的技术，重点关注那些旨在降低合成成本和提高序列保真度的最新进展。开发低成本方法来生产高质量的合成DNA，将通过降低使用成本来促进对更大生物学假设的探索，并有助于缩小DNA读写成本差距。
