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An improved fluorescein diacetate-propidium iodide staining system for assessing microspore and pollen viability at different developmental stages.

作者信息

Tang Jianian, Luo Qiyu, Li Huali, Wu Zichen, Lin Zhansheng, Zhang Sensen, Chen Letian, Liu Yao-Guang

机构信息

Guangdong Basic Research Center of Excellence for Precise Breeding of Future Crops, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, South China Agricultural University, Guangzhou, China.

出版信息

Plant Biotechnol J. 2025 May;23(5):1601-1603. doi: 10.1111/pbi.14607. Epub 2025 Feb 28.

DOI:10.1111/pbi.14607
PMID:40019904
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12018808/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1360/12018808/77ebd8f2a52a/PBI-23-1601-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1360/12018808/77ebd8f2a52a/PBI-23-1601-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1360/12018808/77ebd8f2a52a/PBI-23-1601-g001.jpg

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本文引用的文献

1
Stigma receptors control intraspecies and interspecies barriers in Brassicaceae.柱头受体控制十字花科内种和种间障碍。
Nature. 2023 Feb;614(7947):303-308. doi: 10.1038/s41586-022-05640-x. Epub 2023 Jan 25.
2
Quantitative methods in microscopy to assess pollen viability in different plant taxa.显微镜下评估不同植物分类群花粉活力的定量方法。
Plant Reprod. 2020 Dec;33(3-4):205-219. doi: 10.1007/s00497-020-00398-6. Epub 2020 Oct 29.
3
Rice (Oryza sativa) Protoplast Isolation and Its Application for Transient Expression Analysis.
水稻（Oryza sativa）原生质体分离及其在瞬时表达分析中的应用
Curr Protoc Plant Biol. 2016 Mar;1(2):373-383. doi: 10.1002/cppb.20026.
4
Live-cell fluorescence imaging to investigate the dynamics of plant cell death during infection by the rice blast fungus Magnaporthe oryzae.利用活细胞荧光成像技术研究稻瘟病菌Magnaporthe oryzae感染过程中植物细胞死亡的动态变化。
BMC Plant Biol. 2016 Mar 22;16:69. doi: 10.1186/s12870-016-0756-x.
5
Rice MADS3 regulates ROS homeostasis during late anther development.水稻 MADS3 调控花粉囊晚期发育过程中的 ROS 稳态。
Plant Cell. 2011 Feb;23(2):515-33. doi: 10.1105/tpc.110.074369. Epub 2011 Feb 4.
6
Hybrid male sterility in rice controlled by interaction between divergent alleles of two adjacent genes.水稻中的杂种雄性不育由两个相邻基因的不同等位基因之间的相互作用控制。
Proc Natl Acad Sci U S A. 2008 Dec 2;105(48):18871-6. doi: 10.1073/pnas.0810108105. Epub 2008 Nov 24.
7
Analysis of apoptosis by propidium iodide staining and flow cytometry.通过碘化丙啶染色和流式细胞术分析细胞凋亡。
Nat Protoc. 2006;1(3):1458-61. doi: 10.1038/nprot.2006.238.
8
Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters.通过荧光酯的酶促水解研究活的哺乳动物细胞的膜特性。
Proc Natl Acad Sci U S A. 1966 Jan;55(1):134-41. doi: 10.1073/pnas.55.1.134.