Zheng Hao, Li Sai, Liang Ze-Yin, Feng Ru-Li, Lu Jin-Xing, Dong Yu-Jun, Chen Xiao-Ping
Department of Noscomial Infection, National Institute for Communicable Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing, China.
Department of Laboratory Medicine, HuNan Children's Hospital, Xiangya Medical School, Central South University, Changsha, Hunan, China.
Medicine (Baltimore). 2025 Feb 28;104(9):e41683. doi: 10.1097/MD.0000000000041683.
Due to the severe consequences of viral infection in allogeneic hematopoietic stem cell transplantation (allo-HSCT) patients, the routine use of polymerase chain reaction (PCR) screening of viruses is common in clinic, while the sensitivities of molecular methods are not always sufficient for blood samples. We aimed to increase the detection efficiency of viremia in allo-HSCT patients with M1 bead enrichment. Blood samples of allo-HSCT patients with fever were collected. Simultaneously with analyses of real-time PCR without enrichment, M1 bead enrichment followed by real-time PCR was applied to detect possible viruses in these samples, and metagenomic next-generation sequencing analyses were also applied in 10 samples negative with real-time PCR without enrichment. Various species of viruses were detected with M1 enrichment method. Significantly, some viruses that had not been found by real-time PCR without M1 enrichment were also detected by those with M1 enrichment and verified by metagenomic next-generation sequencing analyses. Furthermore, blood samples enriched with M1 beads had lower Ct values of real-time PCR assay than those that had not been treated. In conclusion, M1 bead enrichment increased the detection efficiency of most viruses in hematological malignancy patients.
由于病毒感染在异基因造血干细胞移植（allo-HSCT）患者中会产生严重后果，临床常规采用聚合酶链反应（PCR）对病毒进行筛查，然而分子方法对血液样本的敏感性并不总是足够的。我们旨在通过M1磁珠富集提高allo-HSCT患者病毒血症的检测效率。收集allo-HSCT发热患者的血液样本。在进行无富集实时PCR分析的同时，应用M1磁珠富集后再进行实时PCR来检测这些样本中可能存在的病毒，并且对10份无富集实时PCR检测为阴性的样本进行了宏基因组二代测序分析。通过M1富集法检测到了多种病毒。值得注意的是，一些未通过无M1富集实时PCR检测到的病毒，也被M1富集实时PCR检测到，并经宏基因组二代测序分析验证。此外，经M1磁珠富集的血液样本实时PCR检测的Ct值低于未处理的样本。总之，M1磁珠富集提高了血液系统恶性肿瘤患者中大多数病毒的检测效率。
