Nordström G, Seeman T, Hasselgren P O
Surgery. 1985 Jun;97(6):679-84.
The effect of allopurinol on protein synthesis, tissue water, and adenine nucleotides in liver tissue during and after a period of liver ischemia was investigated in rats. Ischemia was induced in the left and median liver lobes for 1 hour and experiments were continued for 2 hours after reperfusion. One group of animals (n = 20) received allopurinol (50 mg/kg body weight) intravenously 10 minutes before induction of liver ischemia. Control rats (n = 20) were given a corresponding volume of saline solution. Protein synthesis was measured by determining the rate of amino acid incorporation into protein in incubated liver slices. The reduction of protein synthesis and energy level in liver tissue and the increase of hepatic tissue water were similar in both groups of animals at the end of the ischemic period. During reperfusion the protein synthesis rate was higher and hepatic tissue water was lower in allopurinol-treated animals than in control rats. No significant differences in hepatic adenine nucleotides were found between the two groups of rats during ischemia or after reperfusion. The results demonstrated that improved protein synthesis and reduced tissue water in the postischemic liver after administration of allopurinol were not the result of improved restoration of adenine nucleotides. Inhibited production of oxygen-free radicals might be one mechanism by which allopurinol exerted its beneficial effect after liver ischemia.
在大鼠中研究了别嘌呤醇对肝脏缺血期间及之后肝脏组织中蛋白质合成、组织水分和腺嘌呤核苷酸的影响。在左叶和中叶肝脏诱导缺血1小时，并在再灌注后继续实验2小时。一组动物（n = 20）在诱导肝脏缺血前10分钟静脉注射别嘌呤醇（50 mg/kg体重）。对照大鼠（n = 20）给予相应体积的盐溶液。通过测定孵育的肝切片中氨基酸掺入蛋白质的速率来测量蛋白质合成。在缺血期结束时，两组动物肝脏组织中蛋白质合成和能量水平的降低以及肝组织水分的增加相似。在再灌注期间，别嘌呤醇处理的动物的蛋白质合成速率高于对照大鼠，肝组织水分低于对照大鼠。在缺血期间或再灌注后，两组大鼠的肝脏腺嘌呤核苷酸没有显著差异。结果表明，给予别嘌呤醇后缺血后肝脏中蛋白质合成的改善和组织水分的减少不是腺嘌呤核苷酸恢复改善的结果。抑制氧自由基的产生可能是别嘌呤醇在肝脏缺血后发挥有益作用的一种机制。
