Sabir Shamsa, Hongsibsong Surat, Chuljerm Hataichanok, Parklak Wason, Ounjaijean Sakaewan, Fakfum Puriwat, Kausar Sobia, Kulprachakarn Kanokwan
School of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai, Thailand.
Research Center for Non-infectious Diseases and Environmental Health, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai, Thailand.
PeerJ. 2025 Feb 26;13:e19047. doi: 10.7717/peerj.19047. eCollection 2025.
Exposure to fine particulate matter (PM2.5) is known to increase oxidative stress, impacting health adversely. This study examines the relationship between PM2.5 exposure and oxidative stress biomarkers in Chiang Mai, Thailand.
A pilot prospective observational study was conducted in Samoeng District, Chiang Mai, including 25 healthy participants (age 25-60 years). Urine samples were collected during high (March-April 2023) and low (May-July 2023) PM2.5 seasons. PM2.5 concentrations were monitored daily from the Northern Thailand Air Quality Health Index (NTAQHI) system. Biomarkers analyzed included 1-hydroxypyrene (1-OHP) using high-performance liquid chromatography (HPLC), malondialdehyde (MDA) Spectrophotometry, and 8-epi-prostaglandin F2 (8-epi-PGF2) with Enzyme-linked Immunosorbent Assay (ELISA). Statistical analysis was performed using IBM SPSS Statistics 22.0.
Significant increases in urinary 1-OHP, MDA, and 8-epi-PGF2 were observed during the high PM2.5 season compared to the low season. The mean concentration of PM2.5 was 67 µg/m during high pollution and 7 µg/m during low pollution. Elevated levels of these biomarkers indicate increased oxidative stress associated with higher PM2.5 exposure.
This study highlights a significant association between elevated PM2.5 levels and increased oxidative stress biomarkers in Chiang Mai, Thailand. The findings suggest that exposure to higher concentrations of PM2.5 contributes to oxidative stress, potentially leading to adverse health outcomes.
已知接触细颗粒物（PM2.5）会增加氧化应激，对健康产生不利影响。本研究调查了泰国清迈PM2.5暴露与氧化应激生物标志物之间的关系。
在清迈的沙孟区进行了一项前瞻性试点观察研究，包括25名健康参与者（年龄在25至60岁之间）。在PM2.5浓度高的季节（2023年3月至4月）和低的季节（2023年5月至7月）收集尿液样本。通过泰国北部空气质量健康指数（NTAQHI）系统每日监测PM2.5浓度。分析的生物标志物包括使用高效液相色谱法（HPLC）检测的1-羟基芘（1-OHP）、用分光光度法检测的丙二醛（MDA）以及用酶联免疫吸附测定法（ELISA）检测的8-表前列腺素F2（8-epi-PGF2）。使用IBM SPSS Statistics 22.0进行统计分析。
与低PM2.5季节相比，在高PM2.5季节观察到尿中1-OHP、MDA和8-epi-PGF2显著增加。高污染期间PM2.5的平均浓度为67µg/m，低污染期间为7µg/m。这些生物标志物水平升高表明与较高的PM2.5暴露相关的氧化应激增加。
本研究突出了泰国清迈PM2.5水平升高与氧化应激生物标志物增加之间的显著关联。研究结果表明，接触更高浓度的PM2.5会导致氧化应激，可能导致不良健康后果。
