Huang Yun-Ru, Ding Shinn-Jyh
Institute of Oral Science, Chung Shan Medical University, Taichung City 402, Taiwan.
Department of Stomatology, Chung Shan Medical University Hospital, Taichung City 402, Taiwan.
J Mater Chem B. 2025 Mar 20;13(12):3990-4005. doi: 10.1039/d4tb02656j.
Bone regeneration in the presence of osteoporosis presents a significant challenge in dental and orthopedic surgery. To tackle this issue, researchers have developed strontium-containing biomaterials. However, preventing bacterial infection is also crucial for successful surgical treatment. In this study, we delved deep into the processing to tailor the composition and structure of new strontium silicates with unique properties to address this challenge. We used chemical precipitation to prepare various strontium silicate particles using varying ammonia concentrations and Sr/Si precursor ratios. The L929 cytotoxicity, differentiation of human mesenchymal stem cells (hMSCs), biological function of RAW 264.7 macrophages, and antibacterial activity against and were evaluated. As a result, higher ammonia concentration led to the formation of SrSiO and SrSiO particles with smaller sizes and higher Sr/Si ratios. These particles exhibited increased antibacterial efficacy and radiopacity, promoting cell viability and osteogenic activity of hMSCs and modulating M1/M2 macrophage polarization. In conclusion, the developed strontium silicate demonstrated superior antibacterial activity, exceptional osteogenic properties, and clear visibility during procedures, making it a promising material for bone regeneration and osteoporosis treatment.
在骨质疏松症情况下的骨再生在牙科和矫形外科手术中是一项重大挑战。为解决这一问题，研究人员开发了含锶生物材料。然而，预防细菌感染对于手术治疗的成功也至关重要。在本研究中，我们深入研究了制备过程，以定制具有独特性能的新型硅酸锶的成分和结构，从而应对这一挑战。我们使用化学沉淀法，通过改变氨浓度和Sr/Si前驱体比例来制备各种硅酸锶颗粒。评估了L929细胞毒性、人间充质干细胞（hMSCs）的分化、RAW 264.7巨噬细胞的生物学功能以及对[具体细菌1]和[具体细菌2]的抗菌活性。结果表明，较高的氨浓度导致形成尺寸较小且Sr/Si比例较高的SrSiO₃和Sr₂SiO₄颗粒。这些颗粒表现出增强的抗菌效果和射线不透性，促进hMSCs的细胞活力和成骨活性，并调节M1/M2巨噬细胞极化。总之，所开发的硅酸锶表现出卓越的抗菌活性、出色的成骨性能以及在手术过程中的清晰可视性，使其成为骨再生和骨质疏松症治疗的一种有前景的材料。
