Zhao Yuhui, Zhang Zhihang, Zheng Yi, Bai Huiming, Wu Xiaotong, Yang Yantao, Zhang Junfeng, Yu Chao
Department of Urology, Longhua Hospital Shanghai University of Traditional Chinese Medicine, No. 725, Wanping South Road, Shanghai, 200032, China.
Department of Urology, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, 310006, China.
J Cancer Res Clin Oncol. 2025 Mar 4;151(3):98. doi: 10.1007/s00432-025-06153-6.
Prostate cancer (PCa) is a prevalent malignancy within the male reproductive system that poses a significant threat to patients' lives. The function of long non-coding RNA LINC01128 in PCa progression remains to be elucidated.
The objective was to evaluate the significance of LINC01128 in PCa and to elucidate the underlying mechanisms, thereby identifying a potential target for PCa treatment.
The clinical significance of LINC01128 in PCa was investigated by bioinformatics methods and data analysis. The expression of LINC01128 was quantified using real-time quantitative PCR. The impact of LINC01128 on PCa cell viability and metastasis was evaluated through Cell Counting Kit-8 and Transwell assays. The expression of epithelial-mesenchymal transition markers was analyzed by Western blot analysis. Bioinformatics methods and dual-luciferase reporter assay were employed to explore the mechanisms underlying the role of LINC01128 in PCa progression.
LINC01128 demonstrated significant upregulation in PCa and exhibited a strong correlation with tumor-node-metastasis (TNM) stage, Gleason score, and lymph node metastasis. The upregulation of LINC01128 was found to be linked to a poorer prognosis for PCa. In PCa cells, silencing LINC01128 resulted in the suppression of cell proliferation, migration, and invasion. Furthermore, the knockdown of LINC01128 enhanced the expression of E-cadherin while concurrently repressing the expression of N-cadherin and Vimentin. Mechanistically, the negative regulation of miR-27b-3p by LINC01128 mediated the role of LINC01128 in PCa progression.
In PCa, high expression of LINC01128 may predict patients' unfavorable prognosis. LINC01128 promoted PCa cellular processes by negatively regulating miR-27b-3p.
前列腺癌（PCa）是男性生殖系统中一种常见的恶性肿瘤，对患者生命构成重大威胁。长链非编码RNA LINC01128在PCa进展中的作用仍有待阐明。
评估LINC01128在PCa中的意义并阐明其潜在机制，从而确定PCa治疗的潜在靶点。
通过生物信息学方法和数据分析研究LINC01128在PCa中的临床意义。使用实时定量PCR定量LINC01128的表达。通过细胞计数试剂盒-8和Transwell实验评估LINC01128对PCa细胞活力和转移的影响。通过蛋白质免疫印迹分析上皮-间质转化标志物的表达。采用生物信息学方法和双荧光素酶报告基因实验探讨LINC01128在PCa进展中作用的潜在机制。
LINC01128在PCa中显著上调，且与肿瘤-淋巴结-转移（TNM）分期、 Gleason评分和淋巴结转移密切相关。发现LINC01128的上调与PCa的预后较差有关。在PCa细胞中，沉默LINC01128导致细胞增殖、迁移和侵袭受到抑制。此外，敲低LINC01128增强了E-钙黏蛋白的表达，同时抑制了N-钙黏蛋白和波形蛋白的表达。机制上，LINC01128对miR-27b-3p的负调控介导了LINC01128在PCa进展中的作用。
在PCa中，LINC01128的高表达可能预示患者预后不良。LINC01128通过负调控miR-27b-3p促进PCa细胞进程。
