Gusovsky F, Fawcett J, Javaid J I, Jeffriess H, Sabelli H
Anal Biochem. 1985 Feb 15;145(1):101-5. doi: 10.1016/0003-2697(85)90333-1.
An HPLC procedure for the determination of total phenylacetic acid (PAA) in human plasma is described. After precipitation of plasma proteins with 0.4 N HClO4, the supernatant was hydrolyzed with 1.5 N HCl at 100 degrees C for 5 h, and PAA was extracted with benzene. From the organic layer PAA was back-extracted into 0.5 ml of 0.1 N NaOH. After neutralization with HCl the sample was directly injected onto the HPLC column (C18). An ultraviolet detector at 210 nm was used to monitor PAA. The plasma PAA values for a control population (536.18 +/- 54.99 ng/ml, N = 10) (X +/- SE) obtained by the described method are in agreement with values reported using GC/MS methods. Depressed subjects showed significantly lower values (327.64 +/- 45.44 ng/ml, N = 10), supporting the view that PAA may be a marker for depressive disorders.
本文描述了一种用于测定人血浆中总苯乙酸（PAA）的高效液相色谱法。用0.4N高氯酸沉淀血浆蛋白后，上清液在100℃下用1.5N盐酸水解5小时，然后用苯萃取PAA。将PAA从有机层反萃取到0.5ml 0.1N氢氧化钠中。用盐酸中和后，样品直接注入高效液相色谱柱（C18）。使用210nm的紫外检测器监测PAA。通过所述方法获得的对照组人群（536.18±54.99ng/ml，N = 10）（X±SE）的血浆PAA值与使用气相色谱/质谱法报告的值一致。抑郁症患者的值明显较低（327.64±45.44ng/ml，N = 10），支持PAA可能是抑郁症标志物的观点。
