Abbas Zaheer, Tong Yucui, Zhang Jing, Sammad Abdul, Wang Junyong, Ahmad Baseer, Wei Xubiao, Si Dayong, Zhang Rijun
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Front Immunol. 2025 Feb 18;16:1536694. doi: 10.3389/fimmu.2025.1536694. eCollection 2025.
Natural food-derived bioactive compounds have garnered increasing attention for their potential to modulate immune responses and promote gut health. In particular, compounds like mulberry-derived postbiotics (MDP) may offer novel therapeutic strategies to address inflammation, a key driver of many metabolic disorders.
This study examines the protective effects of MDP against inflammation in LPS-induced mice, using transcriptomic and microbiome analyses to explore underlying mechanisms.
MDP pretreatment alleviates LPSinduced villous atrophy and intestinal barrier damage, promoting recovery of intestinal morphology. Transcriptomic profiling revealed significant changes in gene expression, with 983 upregulated and 1220 downregulated genes in the NC vs LPS comparison, and 380 upregulated and 204 downregulated genes in the LPS vs LPS+MDP comparison. Enrichment analysis using GO and KEGG pathways revealed significant associations with transcriptional regulatory activity, and the NOD-like receptor signaling pathway among the differentially expressed genes. Protein-protein interaction analysis identified key genes involved in inflammation and immune regulation, with hub genes like IL6, CXCL10, and MYD88 in the LPS group and CD74, CIITA, and H2-AB1 in the MDP-treated group.
Microbiome analysis suggested MDP may also influence gut microbiota composition, supporting systemic immune regulation. These findings highlight MDP's potential as a food additive for immune modulation and gut health.
天然食物来源的生物活性化合物因其调节免疫反应和促进肠道健康的潜力而受到越来越多的关注。特别是，像桑椹源后生元（MDP）这样的化合物可能提供新的治疗策略来应对炎症，炎症是许多代谢紊乱的关键驱动因素。
本研究使用转录组学和微生物组分析来探究潜在机制，考察了MDP对脂多糖诱导的小鼠炎症的保护作用。
MDP预处理减轻了脂多糖诱导的绒毛萎缩和肠道屏障损伤，促进了肠道形态的恢复。转录组分析显示基因表达有显著变化，在NC与LPS比较中有983个基因上调和1220个基因下调，在LPS与LPS+MDP比较中有380个基因上调和204个基因下调。使用GO和KEGG通路的富集分析显示与转录调控活性以及差异表达基因中的NOD样受体信号通路有显著关联。蛋白质-蛋白质相互作用分析确定了参与炎症和免疫调节的关键基因，在LPS组中有白细胞介素6、CXC趋化因子配体10和髓样分化因子88等枢纽基因，在MDP处理组中有CD74、Ⅱ类分子反式激活因子和H2-AB1等枢纽基因。
微生物组分析表明MDP也可能影响肠道微生物群组成，支持全身免疫调节。这些发现突出了MDP作为免疫调节和肠道健康食品添加剂的潜力。
