Veenhuis M, Hoogkamer-Te Niet M C, Middelhoven W J
Antonie Van Leeuwenhoek. 1985;51(1):33-43. doi: 10.1007/BF00444226.
Growth of Candida famata and Trichosporon cutaneum on uric acid as the sole source of carbon and nitrogen was associated with the development of a number of microbodies in the cells. Cytochemical staining experiments showed that the organelles contained urate oxidase, a key enzyme of uric acid metabolism, and catalase. Transfer of cells, precultured on glucose or glycerol, into uric acid-containing media indicated that these microbodies originated from the organelles, originally present in the inoculum cells, by growth and division. In urate-grown C. famata the microbodies were frequently observed in large clusters; in both organisms they existed in close association with mitochondria and strands of ER. The organelles lacked crystalline inclusions. In freeze-fractured cells their surrounding membranes showed smooth fracture faces. Exposure of urate-grown cells to glucose-excess conditions led to a rapid inactivation of urate oxidase activity but catalase was only slightly inactivated. Glucose-induced enzyme inactivation was not associated with the degradation of the microbodies present in the cells. Similarly, repression of urate oxidase synthesis by ammonium ions also did not lead to the degradation of peroxisomes.
法塔假丝酵母（Candida famata）和皮状丝孢酵母（Trichosporon cutaneum）在以尿酸作为唯一碳源和氮源的条件下生长时，细胞中会出现许多微体。细胞化学染色实验表明，这些细胞器含有尿酸代谢的关键酶尿酸氧化酶和过氧化氢酶。将在葡萄糖或甘油上预培养的细胞转移到含尿酸的培养基中，结果表明这些微体是通过接种细胞中原本存在的细胞器生长和分裂而来的。在以尿酸生长的法塔假丝酵母中，微体经常以大簇的形式出现；在这两种生物体中，它们都与线粒体和内质网链紧密相连。这些细胞器没有晶体包涵体。在冷冻断裂的细胞中，它们周围的膜呈现出光滑的断裂面。将以尿酸生长的细胞暴露于葡萄糖过量的条件下，会导致尿酸氧化酶活性迅速失活，但过氧化氢酶只是轻微失活。葡萄糖诱导的酶失活与细胞中存在的微体降解无关。同样，铵离子对尿酸氧化酶合成的抑制也不会导致过氧化物酶体的降解。
