Middelhoven W J, De Kievit H, Biesbroek A L
Antonie Van Leeuwenhoek. 1985;51(3):289-301. doi: 10.1007/BF02439938.
Yeast strains utilizing uric acid, adenine, monoamines or diamines as sole source of carbon and energy were isolated from several soil samples by the enrichment culture method. The most common species was Trichosporon cutaneum. Strains of Candida catenulata, C. famata, C. parapsilosis, C. rugosa, Cryptococcus laurentii, Stephanoascus ciferrii and Tr. adeninovorans were also isolated. All strains utilizing uric acid as sole carbon source utilized some primary n-alkyl-l-amines, hydroxyamines or diamines as well. The ascomycetous yeast strains showing these characteristics all belonged to species known to assimilate hydrocarbons. Type strains of hydrocarbon-positive yeast species which were not found in the enrichment cultures generally assimilated putrescine, some type strains also butylamine or pentylamine, but none assimilated uric acid. Methanol-positive species were not isolated. Type strains of methanol-positive and of hydrocarbon-negative species did not assimilate uric acid, butylamine or putrescine. Assimilation of putrescine as sole source of carbon and energy may be a valuable diagnostic criterion in yeast taxonomy.
通过富集培养法，从多个土壤样本中分离出了以尿酸、腺嘌呤、单胺或二胺作为唯一碳源和能源的酵母菌株。最常见的菌种是皮状丝孢酵母。还分离出了链状假丝酵母、法马塔假丝酵母、近平滑假丝酵母、皱落假丝酵母、罗伦隐球酵母、西弗瑞嗜腺酵母和食腺状丝孢酵母的菌株。所有以尿酸作为唯一碳源的菌株也利用一些伯烷基 - l - 胺、羟胺或二胺。表现出这些特征的子囊酵母菌株均属于已知能同化烃类的菌种。在富集培养物中未发现的烃类阳性酵母菌种的模式菌株通常能同化腐胺，一些模式菌株还能同化丁胺或戊胺，但均不能同化尿酸。未分离出甲醇阳性菌种。甲醇阳性和烃类阴性菌种的模式菌株不能同化尿酸、丁胺或腐胺。以腐胺作为唯一碳源和能源进行同化作用，可能是酵母分类学中有价值的诊断标准。
