Bormann C, Sahm H
Arch Microbiol. 1978 Apr 27;117(1):67-72. doi: 10.1007/BF00689353.
Degradation of microbodies in the methanol-utilizing yeast Candida boidinii was mainly studied by electron microscopical observation. The yeast cells precultured on methanol medium contained five to six microbodies per section and showed high activities of alcohol oxidase, catalase, formaldehyde dehydrogenase and formate dehydrogenase. When the precultured cells were transferred into an ethanol medium the number of microbodies and concomitantly the activities of alcohol oxidase and catalase decreased. After 6 h of cultivation microbodies were hardly detected. Also the activity of alcohol oxidase was not measurable and catalase activity was reduced to one tenth, whereas the activities of formaldehyde dehydrogenase and formate dehydrogenase decreased only to about 70%. Experiments with methanol-grown cells transferred into an ethanol medium without nitrogen source indicated that the inactivation of alcohol oxidase and catalase does not require protein synthesis. However, the reappearance of these enzymes is presumably due to de novo protein synthesis as shown by experiments with cycloheximide.
利用电子显微镜观察，主要研究了利用甲醇的酵母博伊丁假丝酵母中微体的降解情况。在甲醇培养基上预培养的酵母细胞，每切片含有五到六个微体，并表现出较高的乙醇氧化酶、过氧化氢酶、甲醛脱氢酶和甲酸脱氢酶活性。当将预培养的细胞转移到乙醇培养基中时，微体数量以及乙醇氧化酶和过氧化氢酶的活性随之降低。培养6小时后，几乎检测不到微体。乙醇氧化酶的活性也无法测量，过氧化氢酶活性降至十分之一，而甲醛脱氢酶和甲酸脱氢酶的活性仅降至约70%。将甲醇培养的细胞转移到无氮源的乙醇培养基中的实验表明，乙醇氧化酶和过氧化氢酶的失活不需要蛋白质合成。然而，如用环己酰亚胺进行的实验所示，这些酶的重新出现可能是由于从头合成蛋白质。
