噬菌体给药及其在模型中对细菌生长抑制的影响：一项研究。
Bacteriophage Dosing and Its Effect on Bacterial Growth Suppression in a Model: An Study.
作者信息
Young Jason, Shariyate Mohammad Javad, Razavi Ahmad Hedayatzadeh, Nazarian Ara, Rodriguez Edward K
机构信息
Harvard Combined Orthopedic Residency Program, Boston, Massachusetts, USA.
Harvard Medical School, Boston, Massachusetts, USA.
出版信息
Phage (New Rochelle). 2024 Dec 18;5(4):223-229. doi: 10.1089/phage.2024.0001. eCollection 2024 Dec.
BACKGROUND
Phages are an emerging therapy in the treatment of prosthetic joint infections, though many challenges remain, including an incomplete understanding of optimal phage dosing.
MATERIALS AND METHODS
We performed an assessment of how phage dosing as measured by multiplicity of infection (MOI) impacts bacterial growth in planktonic and biofilm conditions using a model. ATCC 35984 was combined in planktonic and biofilm forms with phage vB_SepM_Alex at varying concentrations, and growth was monitored via spectrophotometry.
RESULTS
Planktonic bacterial growth was significantly higher when MOI ≤ 0.01 compared with MOI ≥ 10 ( < 0.05). Biofilms with phage dosing at ≤ 10 plaque-forming units (PFU)/mL had significantly greater spectrophotometer readings than those dosed at 10 PFU/mL ( < 0.05).
CONCLUSIONS
Our findings suggest lower, not higher, phage dosing is associated with greater bacterial persistence. Our study helps inform the dosing and delivery of this alternative form of antibiosis.
背景
噬菌体是治疗人工关节感染的一种新兴疗法，不过仍存在许多挑战，包括对最佳噬菌体剂量的认识不全面。
材料与方法
我们使用一个模型评估了以感染复数（MOI）衡量的噬菌体剂量如何影响浮游和生物膜条件下的细菌生长。将ATCC 35984以浮游和生物膜形式与不同浓度的噬菌体vB_SepM_Alex混合，并通过分光光度法监测生长情况。
结果
与MOI≥10相比，当MOI≤0.01时，浮游细菌生长显著更高（P<0.05）。噬菌体剂量≤10噬菌斑形成单位（PFU）/毫升的生物膜的分光光度计读数显著高于剂量为10 PFU/毫升的生物膜（P<0.05）。
结论
我们的研究结果表明，较低而非较高的噬菌体剂量与更强的细菌持久性相关。我们的研究有助于为这种替代抗菌形式的剂量确定和给药方式提供信息。
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