Bolla Nagesh, Garlapati Roopadevi, Vemuri Sayesh, Basam Ram Chowdary, Bolla Lahari, Sahitya Yedla
Department of Conservative Dentistry and Endodontics, Sibar Institute of Dental Sciences, Takkellapadu, Andhra Pradesh, India.
J Conserv Dent Endod. 2025 Feb;28(2):175-181. doi: 10.4103/JCDE.JCDE_764_24. Epub 2025 Feb 3.
Evaluating the efficacy of sonic activation in root canal cleaning using an Endocator adenosine triphosphate (ATP) tester.
The aim of this study was to assess the cleaning efficacy of needle irrigation and sonic activation by measuring ATP values and colony-forming units (CFUs) during root canal procedure.
The design of the study is an study.
Eighty ( = 80) extracted mandibular molars were selected, and distal root canals were enlarged to F2 with ProTaper Gold rotary files. Canals were rinsed with 2 mL of 5.25% sodium hypochlorite (NaOCl), 2 mL of 17% ethylenediaminetetraacetic acid, and 2 mL of distilled water. Samples are sterilized, is inoculated and incubated at 37° for 4 weeks. Baseline ATP values and CFU are measured. Samples are divided into four groups of n = 20 and are irrigated. Group 1:2 mL of distilled water for 30 s. Group 2: 2 mL of distilled water activated with SmartLite Pro EndoActivator (SLP-EA) for 30 s. Group 3: 2 mL of 1% NaOCl for 30 s. Group 4: 2 mL of 1% NaOCl activated with SLP-EA for 30 s. The irrigation procedure was repeated for nine cycles. ATP values and CFU are recorded after each cycle. Friedman, Kruskal-Wallis, Wilcoxon, and Tukey's tests are used for statistical evaluation.
A significant decrease in ATP values and CFU from baseline to residual level is observed in all groups ( ≤ 0.05). Microbial load reduction is significant in Group 4, followed by Groups 2 and 3.
1% NaOCl + SLP-EA showed better efficacy by recording lower ATP values and CFU.
使用Endocator三磷酸腺苷（ATP）测试仪评估声波激活在根管清理中的效果。
本研究的目的是通过在根管治疗过程中测量ATP值和菌落形成单位（CFU）来评估针管冲洗和声波激活的清洁效果。
本研究的设计为一项……研究。
选取80颗拔除的下颌磨牙，使用ProTaper Gold旋转锉将远中根管扩大至F2。根管先用2 mL 5.25%次氯酸钠（NaOCl）冲洗，再用2 mL 17%乙二胺四乙酸冲洗，最后用2 mL蒸馏水冲洗。样本进行灭菌处理，接种并在37℃下培养4周。测量基线ATP值和CFU。样本分为四组，每组n = 20，并进行冲洗。第1组：2 mL蒸馏水冲洗30秒。第2组：用SmartLite Pro根管激活器（SLP-EA）激活的2 mL蒸馏水冲洗30秒。第3组：2 mL 1% NaOCl冲洗30秒。第4组：用SLP-EA激活的2 mL 1% NaOCl冲洗30秒。冲洗过程重复9个周期。每个周期后记录ATP值和CFU。采用Friedman检验、Kruskal-Wallis检验、Wilcoxon检验和Tukey检验进行统计学评估。
所有组从基线到残留水平的ATP值和CFU均显著降低（P≤0.05）。第4组微生物负荷降低显著，其次是第2组和第3组。
1% NaOCl + SLP-EA通过记录较低的ATP值和CFU显示出更好的效果。
