Wang Xiuxiu, Zhao Juan, Gao Mingyang, Wang Tian, Zhang Hongyan
Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Sciences, Shandong Normal University, Jinan 250014, China; College of Sport and Health, Shandong Sport University, Jinan 250102, China.
Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Sciences, Shandong Normal University, Jinan 250014, China.
J Environ Sci (China). 2025 Aug;154:624-634. doi: 10.1016/j.jes.2024.10.011. Epub 2024 Oct 24.
Nano-plastics, emerging pollutants in the environment, have raised global concern due to their widespread presence in daily life and the potential toxicity to human health. Upon entering human body, nano-plastics can readily interact with vascular endothelial cells within the bloodstream, potentially leading to endothelial dysfunction. However, our understanding of the toxic impact of nano-plastics on vascular endothelial cells remains insufficient, and the underlying mechanism are yet to be elucidated. This study investigated the toxicological effects of nano-plastics on EA.hy 926 endothelial cells. Exposure to different types of nano-plastics such as polystyrene (PS), amino-modified PS or carboxyl-modified PS, resulted in suppress cell activity, damage to the cell membrane, oxidative stress and significantly inhibit cell migration. RNA sequencing (RNA-seq) and small RNA-seq analyses revealed that numbers of genes and miRNAs were differentially expressed after nano-plastics treatment. CEBPB, a gene within the inflammation-related tumor necrosis factor signaling pathway, was confirmed to be a target of miR-1908-5p, indicating that nano-plastics induced activation of CEBPB might promote inflammatory injury to vascular endothelial cells. These results enhance our understanding of the biological effects of nano-plastics and their potential impact on inflammation injury.
纳米塑料作为环境中新兴的污染物，因其在日常生活中的广泛存在以及对人类健康的潜在毒性而引发了全球关注。纳米塑料进入人体后，可轻易与血液中的血管内皮细胞相互作用，可能导致内皮功能障碍。然而，我们对纳米塑料对血管内皮细胞毒性影响的了解仍然不足，其潜在机制尚待阐明。本研究调查了纳米塑料对EA.hy 926内皮细胞的毒理学效应。暴露于不同类型的纳米塑料，如聚苯乙烯（PS）、氨基修饰的PS或羧基修饰的PS，会导致细胞活性受到抑制、细胞膜受损、氧化应激，并显著抑制细胞迁移。RNA测序（RNA-seq）和小RNA测序分析表明，纳米塑料处理后有许多基因和微小RNA（miRNA）发生差异表达。炎症相关肿瘤坏死因子信号通路中的基因CEBPB被证实是miR-1908-5p的靶点，这表明纳米塑料诱导的CEBPB激活可能促进血管内皮细胞的炎症损伤。这些结果增进了我们对纳米塑料生物学效应及其对炎症损伤潜在影响的理解。
