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中继投影显微望远镜

Relay-projection microscopic telescopy.

作者信息

Yi Wenjun, Zhu Shuyue, Fu Meicheng, Gu Nan, Qi Junli, Liu Siyu, Zhu Mengjun, Wang Ping, Chen Xin, Zhang Yi, Zhang Hongyu, Xu Yao, Du Junyi, Xiong Peng, Dong Zhaohua, Dong Luobing, Liu Qiong, Li Xiujian

机构信息

College of Science, National University of Defense Technology, Changsha, China.

Xi'an Satellite Control Center, Xi'an, China.

出版信息

Light Sci Appl. 2025 Mar 7;14(1):117. doi: 10.1038/s41377-025-01800-6.

Abstract

The fundamental trade-off between spatial resolution and imaging distance poses a significant challenge for current imaging techniques, such as those used in modern biomedical diagnosis and remote sensing. Here, we introduce a new conceptual method for imaging dynamic amplitude-phase-mixed objects, termed relay-projection microscopic telescopy (rPMT), which fundamentally challenges conventional light collection techniques by employing non-line-of-sight light collection through square-law relay-projection mechanisms. We successfully resolved tiny features measuring 2.76 μm, 22.10 μm, and 35.08 μm for objects positioned at distances of 1019.0 mm, 26.4 m, and 96.0 m, respectively, from single-shot spatial power spectrum images captured on the relay screen; these results demonstrate that the resolution capabilities of rPMT significantly surpass the Abbe diffraction limit of the 25 mm-aperture camera lens at the respective distances, achieving resolution improvement factors of 7.9, 25.4, and 58.2. The rPMT exhibits long-distance, wide-range, high-resolution imaging capabilities that exceed the diffraction limit of the camera lens and the focusing range limit, even when the objects are obscured by a scattering medium. The rPMT enables telescopic imaging from centimeters to beyond hundreds of meters with micrometer-scale resolution using simple devices, including a laser diode, a portable camera, and a diffusely reflecting whiteboard. Unlike contemporary high-resolution imaging techniques, our method does not require labeling reagents, wavefront modulation, synthetic receive aperture, or ptychography scanning, which significantly reduce the complexity of the imaging system and enhance the application practicality. This method holds particular promise for in-vivo label-free dynamic biomedical microscopic imaging diagnosis and remote surveillance of small objects.

摘要

空间分辨率与成像距离之间的基本权衡给当前的成像技术带来了重大挑战,例如现代生物医学诊断和遥感中使用的技术。在此,我们引入了一种用于对动态幅度-相位混合物体进行成像的新概念方法,称为中继投影显微望远镜(rPMT),它通过平方律中继投影机制采用非视线光收集,从根本上挑战了传统的光收集技术。我们通过在中继屏幕上捕获的单次空间功率谱图像,成功分辨出分别位于1019.0毫米、26.4米和96.0米距离处的物体上尺寸为2.76微米、22.10微米和35.08微米的微小特征;这些结果表明,在各自距离下,rPMT的分辨率能力显著超过了25毫米孔径相机镜头的阿贝衍射极限,实现了7.9、25.4和58.2的分辨率提升因子。即使物体被散射介质遮挡,rPMT仍展现出超越相机镜头衍射极限和聚焦范围限制的长距离、宽范围、高分辨率成像能力。rPMT使用包括激光二极管、便携式相机和漫反射白板在内的简单设备,能够以微米级分辨率实现从厘米到数百米以上的望远成像。与当代高分辨率成像技术不同,我们的方法不需要标记试剂、波前调制、合成接收孔径或叠层成像扫描,这显著降低了成像系统的复杂性并增强了应用实用性。该方法在体内无标记动态生物医学显微成像诊断和小物体远程监测方面具有特别的前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/176e/11885427/864e7ea1ce2f/41377_2025_1800_Fig1_HTML.jpg

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