School of Optics and Photonics, Beijing Institute of Technology, Beijing 100081, China.
Sensors (Basel). 2019 Nov 11;19(22):4913. doi: 10.3390/s19224913.
Fourier ptychography microscopy (FPM) is a recently emerged computational imaging method, which combines the advantages of synthetic aperture and phase retrieval to achieve super-resolution microscopic imaging. FPM can bypass the diffraction limit of the numerical aperture (NA) system and achieve complex images with wide field of view and high resolution (HR) on the basis of the existing microscopic platform, which has low resolution and wide field of view. Conventional FPM platforms are constructed based on basic microscopic platform and a scientific complementary metal-oxide-semiconductor (sCMOS) camera, which has ultrahigh dynamic range. However, sCMOS, or even the microscopic platform, is too expensive to afford for some researchers. Furthermore, the fixed microscopic platform limits the space for function expansion and system modification. In this work, we present a simply equipped FPM platform based on an industrial camera and telecentric objective, which is much cheaper than sCMOS camera and microscopic platform and has accurate optical calibration. A corresponding algorithm was embedded into a conventional FP framework to overcome the low dynamic range of industrial cameras. Simulation and experimental results showed the feasibility and good performance of the designed FPM platform and algorithms.
傅里叶叠层光显微术（Fourier ptychography microscopy，FPM）是一种新兴的计算成像方法，它结合了合成孔径和相位恢复的优势，实现了超分辨率显微镜成像。FPM 可以在现有的显微镜平台基础上，绕过数值孔径（numerical aperture，NA）系统的衍射极限，实现具有大视场和高分辨率（high resolution，HR）的复杂图像，而传统的显微镜平台具有低分辨率和大视场。传统的 FPM 平台是基于基本的显微镜平台和具有超高动态范围的科学互补金属氧化物半导体（scientific complementary metal-oxide-semiconductor，sCMOS）相机构建的。然而，sCMOS 甚至显微镜平台对于一些研究人员来说都过于昂贵。此外，固定的显微镜平台限制了功能扩展和系统修改的空间。在这项工作中，我们提出了一种基于工业相机和远心物镜的简单配置的 FPM 平台，它比 sCMOS 相机和显微镜平台便宜得多，并且具有精确的光学校准。我们将相应的算法嵌入到传统的 FP 框架中，以克服工业相机的低动态范围。模拟和实验结果表明，所设计的 FPM 平台和算法具有可行性和良好的性能。
