Barrios Andana, Gaggion Nicolas, Mansilla Natanael, Blein Thomas, Sorin Céline, Lucero Leandro, Ferrante Enzo, Crespi Martin, Ariel Federico
Institute of Plant Sciences Paris Saclay IPS2, CNRS, INRA, Université Evry, Université Paris-Saclay, Bâtiment 630, Orsay, 91405, France.
Institute of Plant Sciences Paris-Saclay IPS2, Université de Paris, Bâtiment 630, Orsay, 91405, France.
Plant J. 2025 Mar;121(5):e70016. doi: 10.1111/tpj.70016.
Root developmental plasticity relies on transcriptional reprogramming, which largely depends on the activity of transcription factors (TFs). NF-YA2 and NF-YA10 (nuclear factor A2 and A10) are downregulated by the specific miRNA isoform miR169defg. Here, we analyzed the role of the Arabidopsis thaliana TF NF-YA10 in the regulation of lateral root (LR) development. Plants expressing a version of NF-YA10 resistant to miR169 cleavage showed a perturbation in the LR gravitropic response. By extracting several features of root architecture using an improved version of the ChronoRoot deep-learning-based phenotyping system, we uncovered that these plants showed a differential angle of LRs over time when compared to Col-0. Detailed phenotyping of root growth dynamics revealed that NF-YA10 misregulation modulates the area explored by Arabidopsis roots. Furthermore, we found that NF-YA10 directly regulates LAZY genes, which were previously linked to gravitropism, by targeting their promoter regions. Hence, the TF NF-YA10 is a new element in the control of LR bending and root system architecture.
根发育可塑性依赖于转录重编程，这在很大程度上取决于转录因子（TFs）的活性。NF-YA2和NF-YA10（核因子A2和A10）被特定的miRNA亚型miR169defg下调。在这里，我们分析了拟南芥转录因子NF-YA10在侧根（LR）发育调控中的作用。表达对miR169切割具有抗性的NF-YA10版本的植物在LR向重力性反应中表现出扰动。通过使用基于深度学习的ChronoRoot表型系统的改进版本提取根系结构的几个特征，我们发现与Col-0相比，这些植物的LR随着时间的推移呈现出不同的角度。对根生长动态的详细表型分析表明，NF-YA10调控异常会调节拟南芥根探索的面积。此外，我们发现NF-YA10通过靶向LAZY基因的启动子区域直接调控这些先前与向重力性相关的基因。因此，转录因子NF-YA10是控制LR弯曲和根系结构的一个新元件。
