Whole genome sequence of an edible mushroom Strobilomyces alpinus (Boletaceae).

Strobilomyces alpinus is a unique and significant mushroom endemic to southwestern and central China, characterized by its exclusive subalpine distribution and a strong host preference for Abies spp. The biological and genetic studies of this mushroom are scarce, which significantly hinders research on molecular breeding and evolutionary patterns. In this study, we report the de novo sequencing and assembly of the S. alpinus genome using the DNBSEQ-T7 and the third-generation Pacific Biosciences sequencing platform. The total genome size was approximately 58.75 Mb, with a GC content of 54.87%. The genome assembly produced 68 contigs, with an N50 length of 4.03 Mb. The genome comprises 11,761 annotated protein-coding genes, including 813 CAZyme-coding genes, 182 Cytochrome P450 genes, and 1,821 candidate pathogenicity-related genes. The non-coding RNA prediction results indicated the presence of 532 rRNAs, 62 small RNAs, and 98 tRNAs in the S. alpinus genome. Notably, there is a high degree of repetition (44.28%) within the S. alpinus genome. Additionally, we identified 16 secondary metabolite gene clusters, including 7 NRPS-like clusters, 5 terpene clusters, 1 fungal-RiPP-like cluster, 1 RiPP-like cluster, 1 T1PKS cluster, and 1 T1PKS-NRPS linkage gene cluster. Several important metabolic pathways, including terpenoid backbone biosynthesis, porphyrin metabolism, and folate biosynthesis, have been elucidated. The annotated whole-genome sequence of S. alpinus can serve as a reference for investigations of bioactive compounds with medicinal value and for commercial production.