Kennell Amanda, Shivers Olivia, Chatterton Ranoah, Stanishevsky Andrei
Center for Materials and Manufacturing Sciences, Department of Chemistry and Physics, Troy University, Troy, AL, 36082, USA.
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL, 35294, USA.
Macromol Rapid Commun. 2025 Jul;46(13):e2500036. doi: 10.1002/marc.202500036. Epub 2025 Mar 8.
Electrospun fish gelatin (FGel) nanofibers (NF) mimic the natural bodies extracellular matrix's (ECM) structure and are an attractive material for many biomedical applications. However, FGel poor mechanical properties and rapid dissolution in an aqueous media paired with usually low productivity of the typical electrospinning process necessitate further effort in overcoming these issues. In this study, alternating field electrospinning (AFES) fabricates cold water fish skin gelatin nanofibrous materials (FGel NFM) with up to 10 wt.% Dextran (DEX) or acetyl glucosamine (AGA) from pure aqueous solutions at process productivity of 7.92-8.90 g∙h. Thermal crosslinking of as-spun materials resulted in FGel-based NFM with 125-325 nm fiber diameters. DEX (MW500k and MW75k) and AGA additives cause different effects on FGel fiber diameters, structure, tensile and degradation behavior, and in vitro performance. All tested materials reveal favorable, but not the same, cellular response through the formation of a confluent layer on the NFM surface regardless of the fibers' composition despite the significant difference in FGel NFM structure and properties. Results show that AFES and thermal crosslinking of FGel-based NFM can lead to a sustainable "green" fabrication technology of mono- and polysaccharide modified FGel-based NFM scaffolds with the parameters attuned to targeted biomedical applications.
静电纺丝鱼明胶（FGel）纳米纤维（NF）模仿天然细胞外基质（ECM）的结构，是许多生物医学应用中具有吸引力的材料。然而，FGel机械性能差、在水性介质中快速溶解，再加上典型静电纺丝工艺通常较低的生产率，需要进一步努力克服这些问题。在本研究中，交变电场静电纺丝（AFES）从纯水溶液中制备了含有高达10 wt.% 葡聚糖（DEX）或乙酰氨基葡萄糖（AGA）的冷水鱼皮明胶纳米纤维材料（FGel NFM），工艺生产率为7.92 - 8.90 g∙h。初纺材料的热交联产生了纤维直径为125 - 325 nm的基于FGel的NFM。DEX（MW500k和MW75k）和AGA添加剂对FGel纤维直径、结构、拉伸和降解行为以及体外性能有不同影响。所有测试材料通过在NFM表面形成汇合层显示出良好但不同的细胞反应，无论纤维组成如何，尽管FGel NFM结构和性能存在显著差异。结果表明，基于FGel的NFM的AFES和热交联可以导致一种可持续的“绿色”制造技术，用于制备单糖和多糖改性的基于FGel的NFM支架，其参数可根据目标生物医学应用进行调整。
