Perewari Doubra Otis, Otokunefor Kome, Agbagwa Obakpororo Ejiro
Department of Microbiology, Niger Delta University, P.M.B. 071, Amassoma, Bayelsa, Nigeria.
Department of Microbiology, University of Port Harcourt, P.M.B. 5323, Port Harcourt, Nigeria.
Int J Microbiol. 2022 Jul 9;2022:9192424. doi: 10.1155/2022/9192424. eCollection 2022.
Monitoring the occurrence of tetracycline resistance and its determinants in both clinical and nonclinical settings is essential in understanding the role played by continuous usage of this drug in animal husbandry and the withdrawal of this drug from clinical practice. Limited information is available on this from our locale. This study, therefore, set out to explore the occurrence of specific tetracycline-resistant genes in from clinical and nonclinical sources in Rivers State, Nigeria.
Two hundred clinical and nonclinical samples were analyzed for the presence of using standard phenotypic and genotypic tests. Susceptibility testing was carried out using the Kirby-Bauer disc diffusion method, and specific tetracycline-resistant genes ( and ) were assayed.
Results showed that stool samples had the highest occurrence of (39, 78%), and soil had the lowest (13, 26%). Tetracycline resistance was observed in 80.7% of total isolates. The genes were the most commonly occurring ( = 80, 89.9%) detected in confirmed isolates, and , the least commonly occurring ( = 16,18%) of isolates. The combined presence of tetA-tetM was the highest ( = 14, 15.7%), followed by tetA-tetB ( = 13, 14.8%).
The present study reports on the occurrence and distribution of four tetracycline-resistant determinants in from clinical and nonclinical sources in Rivers State, Nigeria. The high-level occurrence of the most commonly occurring tetracycline gene even in nonclinical isolates could be indicative of a potential reservoir of this resistance. And, this could limit the reintroduction of tetracycline even in combination therapy.
监测四环素耐药性及其决定因素在临床和非临床环境中的发生情况，对于理解该药物在畜牧业中的持续使用以及从临床实践中停用所起的作用至关重要。我们当地关于这方面的信息有限。因此，本研究旨在探讨尼日利亚河流州临床和非临床来源中特定四环素耐药基因的发生情况。
使用标准的表型和基因型测试分析200份临床和非临床样本中[具体耐药基因未给出]的存在情况。采用 Kirby-Bauer 纸片扩散法进行药敏试验，并检测特定的四环素耐药基因（[具体基因未给出]和[具体基因未给出]）。
结果显示，粪便样本中[具体耐药基因未给出]的发生率最高（39份，78%），土壤样本中最低（13份，26%）。在所有分离株中，80.7%观察到四环素耐药性。[具体基因未给出]基因是在确诊的[具体耐药菌未给出]分离株中最常见的（80份，89.9%），而[具体基因未给出]是分离株中最不常见的（16份，18%）。tetA - tetM 的联合存在率最高（14份，15.7%），其次是 tetA - tetB（13份，14.8%）。
本研究报告了尼日利亚河流州临床和非临床来源中四种四环素耐药决定因素的发生和分布情况。即使在非临床分离株中，最常见的四环素基因的高发生率也可能表明这种耐药性的潜在储存库。而且，这可能会限制四环素即使在联合治疗中的重新引入。
