Aberkane Chahrazed, Messaï Ahmed, Messaï Chafik Redha, Boussaada Tarek
Department of Agricultural Sciences, DEDSPAZA Laboratory, Mohamed-Khider University, Biskra, Algeria.
Department of Agricultural Sciences, PIARA Laboratory, Mohamed-Khider University, Biskra, Algeria.
Vet World. 2023 Mar;16(3):449-454. doi: 10.14202/vetworld.2023.449-454. Epub 2023 Mar 16.
Avian pathogenic (APEC) is the causative agent of colibacillosis, one of the most prevalent bacterial diseases responsible for significant economic losses in the poultry industry worldwide. This study aimed to assess the antimicrobial resistance (AMR) patterns of APEC isolates recovered from poultry in east Algeria and estimate the prevalence of extended-spectrum β-lactamase (ESBL)-producing isolates.
In the slaughterhouse of Batna City (Algeria), livers indicating colibacillosis were sampled from 204 suspected carcasses with growth retardation and generalized congestion. isolation and identification were performed on MacConkey agar using conventional methods and the API 20E system. Antimicrobial resistance susceptibility was tested by the disk diffusion method according to the Clinical Laboratory Standards Institute Guidelines. Extended-spectrum β-lactamase detection was carried out using the double-disk confirmation test.
One hundred sixty isolates were recovered (one isolate per sample). Avian pathogenic isolates showed high levels of resistance to ampicillin and tetracycline (100%), nalidixic acid (95%), ofloxacin (93.75%), doxycycline (91.87), ciprofloxacin (87.50%), trimethoprim/sulfamethoxazole (62.50%), gentamycin (32.50%), chloramphenicol (27.50%), amoxicillin/clavulanic acid (16.25%), colistin (14.37%), and nitrofurantoin (10.62%). All strains were multidrug-resistant to at least three antibiotics, and more than half (52.52%) of the isolates were resistant to at least seven antibiotics. All isolates were susceptible to ceftriaxone, ceftazidime, and aztreonam. Two isolates were ESBL producers (1.25%).
Avian pathogenic resistance to most antimicrobial agents used in poultry may lead to antimicrobial therapy failure.
禽致病性大肠杆菌（APEC）是禽大肠杆菌病的病原体，禽大肠杆菌病是全球家禽业中最普遍的细菌性疾病之一，会造成重大经济损失。本研究旨在评估从阿尔及利亚东部家禽中分离出的APEC菌株的抗菌药物耐药性（AMR）模式，并估计产超广谱β-内酰胺酶（ESBL）菌株的流行率。
在巴特纳市（阿尔及利亚）的屠宰场，从204具疑似生长发育迟缓且全身充血的尸体上采集显示有大肠杆菌病的肝脏样本。使用常规方法和API 20E系统在麦康凯琼脂上进行分离和鉴定。根据临床实验室标准协会指南，采用纸片扩散法检测抗菌药物敏感性。使用双纸片确证试验进行超广谱β-内酰胺酶检测。
共分离出160株菌株（每个样本一株）。禽致病性大肠杆菌菌株对氨苄西林和四环素的耐药率较高（100%），对萘啶酸的耐药率为95%，对氧氟沙星的耐药率为93.75%，对强力霉素的耐药率为91.87%，对环丙沙星的耐药率为87.50%，对甲氧苄啶/磺胺甲恶唑的耐药率为62.50%，对庆大霉素的耐药率为32.50%，对氯霉素的耐药率为27.50%，对阿莫西林/克拉维酸的耐药率为16.25%，对黏菌素的耐药率为14.37%，对呋喃妥因的耐药率为10.62%。所有菌株对至少三种抗生素具有多重耐药性，超过一半（52.52%）的分离株对至少七种抗生素耐药。所有分离株对头孢曲松、头孢他啶和氨曲南敏感。有两株分离株产ESBL（1.25%）。
禽致病性大肠杆菌对家禽中使用的大多数抗菌药物耐药可能导致抗菌治疗失败。
