Coon J S, Watkins J R, Pauli B U, Weinstein R S
Cancer Res. 1985 Jul;45(7):3014-21.
Previous immunohistological studies showed a relationship between expression of blood group-related antigens (BG-Ag) and invasive potential in human urinary bladder carcinoma, but the marked variability of antigen staining within many individual tumors has obscured the biological basis of this finding. We studied the expression of the A, H, and T (Thomsen-Friedenreich) BG-Ag by flow cytometry in a human bladder carcinoma cell line (647V) using fluoresceinated BG-Ag-specific lectins (Dolichos bifloris, Ulex europaeus, and Arachis hypogaea). Cell cycle compartments were quantitated by flow cytometry using propidium iodide staining. Expression of all three antigens was highly variable, but staining for each antigen produced a distinct profile. T antigen expression appeared independent of A or H antigen expression. Cell populations sorted by T antigen expression showed heritable antigenic differences persistent over many weeks in culture. However, much of the T antigen variability was nonheritable, since the stable staining profiles of the sorted cells were intermediate between the parental and the profiles obtained immediately after sorting. The nonheritable antigenic variation did not appear entirely explainable by cell size or cell cycle fluctuation. These results were confirmed by isolating 64 clones from the dim part of the T antigen staining profile, 19 of which had a persistently dim phenotype. The variability of BG-Ag expression by human bladder carcinoma cells in vitro may explain the staining patterns observed in the study of antigen expression in resected human bladder carcinomas.
以往的免疫组织学研究表明，血型相关抗原（BG-Ag）的表达与人类膀胱癌的侵袭潜能之间存在关联，但许多单个肿瘤内抗原染色的显著变异性掩盖了这一发现的生物学基础。我们使用荧光素标记的BG-Ag特异性凝集素（双花扁豆、欧洲荆豆和落花生），通过流式细胞术研究了人膀胱癌细胞系（647V）中A、H和T（汤姆森-弗里德赖希）BG-Ag的表达。使用碘化丙啶染色通过流式细胞术对细胞周期区室进行定量。所有三种抗原的表达高度可变，但每种抗原的染色产生了独特的图谱。T抗原的表达似乎独立于A或H抗原的表达。按T抗原表达分选的细胞群体在培养数周后显示出可遗传的抗原差异。然而，大部分T抗原的变异性是不可遗传的，因为分选细胞的稳定染色图谱介于亲代和分选后立即获得的图谱之间。不可遗传的抗原变异似乎不能完全由细胞大小或细胞周期波动来解释。从T抗原染色图谱的暗淡部分分离出64个克隆，其中19个具有持续暗淡的表型，从而证实了这些结果。人膀胱癌细胞体外BG-Ag表达的变异性可能解释了在切除的人膀胱癌抗原表达研究中观察到的染色模式。
