Ramachandran Hariprasad, Kuriakose Feby, Joseph Shiny, Anoop V N, Thushar Balan K
Department of Dentistry, Neyyar Medicity Hospital, Kattakada, Trivandrum, Kerala, India.
Department of Endodontics, Mar Baselios Dental College, Affiliated to Kerala University of Health Sciences, Ernakulam, Kerala, India.
J Pharm Bioallied Sci. 2024 Dec;16(Suppl 5):S4339-S4343. doi: 10.4103/jpbs.jpbs_526_24. Epub 2025 Jan 30.
Most methods for retrieving fractured instruments have consequences that may affect treatment outcomes. A better outcome can be achieved with electrochemical dissolution without mechanical injury.
Several electrochemical dissolution solutions are being used including artificial saliva, Tyrode's solution, and sodium fluoride (NaF). These electrolytes were prepared and their respective electrochemical characterization was done before conducting the assay. The present in vitro study compares the possible cytotoxic effect and viability of periodontal ligament cells in artificial saliva, Tyrode's solution, and NaF using an MTT assay.
The comparative analyses of the cell viability and toxicity effects of the electrolytes considered are reported. It was observed that the Artificial saliva (before dissolution: 84.10±11.32; after dissolution: 69.1±11.32) and Tyrode's solution (before dissolution: 77.30±4.11; after dissolution: 61.3±4.11) showed higher cell viability percentages and comparatively reduced toxicity levels before and after dissolution compared to NaF (before dissolution: 36.01±5.27; after dissolution: 11.01±5.27). Statistical analysis of the results observed before and after dissolution provided a significant difference (<0.05).
The artificial saliva and the Tyrode's solution may be considered as a potential electrochemical dissolution solution during the surgery to achieve a better outcome.
大多数取出折断器械的方法都会产生可能影响治疗效果的后果。采用电化学溶解且无机械损伤的方法可获得更好的效果。
正在使用几种电化学溶解溶液，包括人工唾液、台氏液和氟化钠（NaF）。在进行实验前，制备了这些电解质并对其各自的电化学特性进行了测定。本体外研究使用MTT法比较人工唾液、台氏液和NaF中牙周膜细胞的可能细胞毒性作用和活力。
报告了所考虑的电解质的细胞活力和毒性作用的比较分析。观察到，与NaF（溶解前：36.01±5.27；溶解后：11.01±5.27）相比，人工唾液（溶解前：84.10±11.32；溶解后：69.1±11.32）和台氏液（溶解前：77.30±4.11；溶解后：61.3±4.11）在溶解前后显示出更高的细胞活力百分比和相对较低的毒性水平。对溶解前后观察到的结果进行统计分析，结果有显著差异（<0.05）。
人工唾液和台氏液在手术期间可被视为潜在的电化学溶解溶液，以获得更好的效果。
