Almalki Sami G, Al-Ghamdi Youssef O, Algethami Faisal K, Daoush Walid M, Alrfaei Bahauddeen M, Alanazi Maisa, Alqurashi Yaser E, Jabli Mahjoub
Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Majmaah, 11952, Saudi Arabia.
Department of Chemistry, College of Science Al-Zulfi, Majmaah University, Al-Majmaah, 11952, Saudi Arabia.
Int J Nanomedicine. 2025 Mar 3;20:2593-2607. doi: 10.2147/IJN.S498894. eCollection 2025.
Previous studies on synthesis and cytotoxicity effect of copper (Cu) and magnetite (FeO) nanodots against kidney and lung cancer cells Lines.
Investigation of the effects of Cu and FeO nanodots on the reduction in viability of lung cancer (A549) and human embryonic kidney (HEK239T) cells lines.
Cu and FeO nanodots/Gum Arabic (GA) hybrids were chemically synthesized, characterized, and assessed by MTT and flow cytometry for their cytotoxicity against A549 and HEK239T cells lines.
Novel hybrids of Cu and FeO nanodots were effectively synthesized by chemical deposition method in combination with GA stabilizing agent. The stabilized Cu and FeO nanodots by GA have median particle size of 4.7 nm and 7 nm respectively. Furthermore, the biological activities of Cu nanodots/ GA hybrids showed a strong reduction in viability for both A549 and HEK239T cells at 50 and 100 ng/µL, owing to their small size and high surface-area-to-volume ratio compared to FeO nanoparticles. Pre-apoptosis effect of the Cu nanodots/ GA hybrid on the treated A549 cells were 40% for 0.1 ng/µL, 91.4% for 1 ng/µL, 88% for 50 ng/ µL, and 87.7% for 100 ng/µL. However, in the treated human embryonic kidney (HEK293T) cells, the pre-apoptosis was found under different concentrations conditions of 89.9% for 0.1 ng/µL, 94.2% for 1 ng/µL, 76.2% for 50 ng/µL and 70.5% for 100 ng/µL. After treatment of both A549 and HEK293T cells with 100 ng/µL of Cu nanodots/GA hybrid, many cell deaths and reformed nanoparticles crystals were observed.
Cu nanodots/GA hybrid strongly reduce the viability of both A549 and HEK239T cells at 50 and 100 ng/µL. However, FeO nanodots/GA hybrid did not show any significant effect.
先前关于铜（Cu）和磁铁矿（FeO）纳米点对肾癌细胞系和肺癌细胞系的合成及细胞毒性作用的研究。
研究Cu和FeO纳米点对肺癌（A549）细胞系和人胚肾（HEK239T）细胞系活力降低的影响。
化学合成Cu和FeO纳米点/阿拉伯树胶（GA）杂化物，对其进行表征，并通过MTT法和流式细胞术评估它们对A549和HEK239T细胞系的细胞毒性。
通过化学沉积法结合GA稳定剂有效地合成了新型的Cu和FeO纳米点杂化物。由GA稳定的Cu和FeO纳米点的中位粒径分别为4.7纳米和7纳米。此外，Cu纳米点/GA杂化物的生物活性表明，在50和100纳克/微升时，A549和HEK239T细胞的活力均大幅降低，这是因为与FeO纳米颗粒相比，它们尺寸小且表面积与体积比高。Cu纳米点/GA杂化物对处理后的A549细胞的凋亡前期效应在0.1纳克/微升时为40%，1纳克/微升时为91.4%，50纳克/微升时为88%，100纳克/微升时为87.7%。然而，在处理后的人胚肾（HEK293T）细胞中，在不同浓度条件下发现凋亡前期情况为：0.1纳克/微升时为89.9%，1纳克/微升时为94.2%，50纳克/微升时为76.2%，100纳克/微升时为70.5%。用100纳克/微升的Cu纳米点/GA杂化物处理A549和HEK293T细胞后，观察到许多细胞死亡并形成了纳米颗粒晶体。
Cu纳米点/GA杂化物在50和100纳克/微升时能显著降低A549和HEK239T细胞的活力。然而，FeO纳米点/GA杂化物未显示出任何显著效果。
