Kiani Roghayeh, Mojgani Naheed, Kobarfard Farzad, Saffarian Parvaneh, Ayatollahi Seyed Abdulmajid, Khoramjouy Mona
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Biotechnology Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.
Iran J Pharm Res. 2024 Dec 25;23(1):e148158. doi: 10.5812/ijpr-148158. eCollection 2024 Jan-Dec.
is a significant contributor to a range of gastrointestinal conditions, with conventional treatment methods primarily relying on antibiotics. However, the rise of antibiotic-resistant strains has necessitated the exploration of alternative therapeutic approaches.
To determine the antibacterial potential of probiotic bacteria ( BLRH 260 and ) and four propolis extracts against and to analyze their impacts on body weight index and histopathological changes in -challenged Wistar rats.
The inhibitory effects of probiotic bacteria ( BLRH 260 and ) and propolis extracts on the growth of were evaluated using an agar well diffusion assay. In vivo analysis involved fifty-four male Wistar rats (200 - 250 g) infected with an suspension (10 CFU/mL) and orally administered propolis or probiotics (10 CFU/mL) via gavage for 21 days. The effects of different treatments on body weight and histopathological changes in gastric tissue samples were assessed, and the results were statistically analyzed.
The tested propolis extracts and the supernatant fluids from the mentioned probiotic strains showed significant antibacterial activity against in the agar well diffusion assay, with notable variations. In vivo, the findings demonstrated that oral administrations of propolis and probiotics, either separately or in combination, led to significant increases in body weight and amelioration of histopathological changes in gastric tissue samples, particularly in terms of erosion depth, hemorrhagic inflammation, and apoptosis in the infected animals. Histopathological differences between antibiotic-treated animals and those receiving other treatments were observed, with significant differences.
The results of this study underscore the potential therapeutic benefits of propolis and probiotics in addressing -induced gastropathy. Additional research is necessary to clarify the mechanisms involved and to refine dosage and treatment protocols for optimal effectiveness.
是一系列胃肠道疾病的重要促成因素，传统治疗方法主要依赖抗生素。然而，抗生素耐药菌株的出现使得探索替代治疗方法成为必要。
确定益生菌（BLRH 260和）和四种蜂胶提取物对的抗菌潜力，并分析它们对感染的Wistar大鼠体重指数和组织病理学变化的影响。
采用琼脂孔扩散法评估益生菌（BLRH 260和）和蜂胶提取物对生长的抑制作用。体内分析涉及54只雄性Wistar大鼠（200 - 250克），用悬浮液（10CFU/mL）感染，通过灌胃口服给予蜂胶或益生菌（10CFU/mL），持续21天。评估不同处理对胃组织样本体重和组织病理学变化的影响，并对结果进行统计分析。
在琼脂孔扩散试验中，测试的蜂胶提取物和上述益生菌菌株的上清液对显示出显著的抗菌活性，存在显著差异。在体内，研究结果表明，单独或联合口服蜂胶和益生菌可导致体重显著增加，并改善胃组织样本的组织病理学变化，特别是在感染动物的糜烂深度、出血性炎症和细胞凋亡方面。观察到抗生素治疗动物与接受其他治疗动物之间的组织病理学差异，差异显著。
本研究结果强调了蜂胶和益生菌在解决诱导的胃病方面的潜在治疗益处。需要进一步研究以阐明其中涉及的机制，并完善剂量和治疗方案以实现最佳效果。
