Laboratory of Antimicrobial Testing, Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia 38405318, MG, Brazil.
Complex of Animal Facilities, Federal University of Uberlândia, Uberlândia 38405315, MG, Brazil.
Molecules. 2022 Oct 27;27(21):7310. doi: 10.3390/molecules27217310.
is a Gram-negative, microaerophilic, curved-rod, flagellated bacterium commonly found in the stomach mucosa and associated with different gastrointestinal diseases. With high levels of prevalence worldwide, it has developed resistance to the antibiotics used in its therapy. Brazilian red propolis has been studied due to its biological properties, and in the literature, it has shown promising antibacterial activities. The aim of this study was to evaluate anti- from the crude hydroalcoholic extract of Brazilian red propolis (CHEBRP). For this, in vitro determination of the minimum inhibitory and bactericidal concentration (MIC/MBC) and synergistic activity and in vivo, microbiological, and histopathological analyses using Wistar rats were carried out using CHEBRP against strains (ATCC 46523 and clinical isolate). CHEBRP presented MIC/MBC of 50 and 100 μg/mL against strains (ATCC 43526 and clinical isolate, respectively) and tetracycline MIC/MBC of 0.74 µg/mL. The association of CHEBRP with tetracycline had an indifferent effect. In the stomach mucosa of rats, all treatments performed significantly decreased the number of , and a concentration of 300 mg/kg was able to modulate the inflammatory response in the tissue. Therefore, CHEBRP showed promising anti- in in vitro and in vivo assays.
幽门螺杆菌是一种革兰氏阴性、微需氧、弯曲杆菌、鞭毛细菌，通常存在于胃黏膜中，与不同的胃肠道疾病有关。由于其在全球的高患病率，它已经对用于治疗的抗生素产生了耐药性。巴西红蜂胶因其生物特性而受到研究，在文献中显示出有希望的抗菌活性。本研究旨在评估巴西红蜂胶粗水醇提取物（CHEBRP）的抗活性。为此，使用 CHEBRP 对 株（ATCC 46523 和临床分离株）进行了体外最小抑菌和杀菌浓度（MIC/MBC）测定以及协同活性和体内微生物学和组织病理学分析，Wistar 大鼠。CHEBRP 对 株（ATCC 43526 和临床分离株，分别）和四环素 MIC/MBC 的 MIC/MBC 为 50 和 100 μg/mL。CHEBRP 与四环素的联合使用具有中性作用。在大鼠胃黏膜中，所有治疗均显著降低了 的数量，浓度为 300mg/kg 能够调节组织中的炎症反应。因此，CHEBRP 在体外和体内试验中均显示出有希望的抗活性。
