Ji Renlong, Xu Yanchang, Li Kang, Jiang Wen, Li Yuan, Zeng Jianwei, Li Wei, Wang Caiji, Zhao Zeqi, Li Yalan, Konduru Naveena, Liu Wen, Qiao Yuehua, Li Xuanyi
Department of Otorhinolaryngology-Head and Neck Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Institute of Audiology and Balance Science, Xuzhou Medical University, Xuzhou, China.
Front Neurol. 2025 Feb 24;16:1430522. doi: 10.3389/fneur.2025.1430522. eCollection 2025.
To investigate the effects of Zexie decoction on vestibular and auditory function in guinea pigs with endolymphatic hydrolysis induced by desmopressin. Methods: Sixty guinea pigs were randomly and evenly divided into four groups, each group has 15 guinea pigs: normal control group, DDAVP group, DDAVP modeling combined with Zexie Decoction group, and DDAVP combined with Double Zexie group. At 7 and 14 days, bone-conducted cervical vestibular evoked myogenic potential tests, auditory brainstem responses, and distortion-product otoacoustic emissions were conducted on each group of guinea pigs to evaluate their vestibular and auditory function quantitatively. After functional testing, the outer hair cells were observed by scanning electron microscope. On day 14, one guinea pig was randomly selected from both the normal control group and DDAVP group to verify the successful establishment of the model using gadolinium-enhanced magnetic resonance imaging of the inner ear.
We conducted BC-cVEMP, ABR, and DPOAE tests on guinea pigs, and the results showed that DDAVP did affect vestibular function and hearing in guinea pigs. Analyses were performed from those results that were statistically significant, Zexie Decoction improved DDAVP-induced vestibular dysfunction and hearing loss dose-dependently, though complete reversal was not achieved. About scanning electron microscopy, outer hair cells of the DDAVP group showed significant loss and cilia lodging, however, treatment with Zexie decoction can alleviate the loss of outer hair cells and the lodging of cilia. When the outer hair cells were exposed to DDAVP for a long time, the improvement effect of Zexie decoction was not as obvious as before.
The extent of improvement correlates with the concentration and dosage of Zexie Decoction. Even at double the dosage, Zexie Decoction only partially mitigates the decline in vestibular and auditory function induced by DDAVP, falling short of complete reversal.
探讨泽泻汤对去氨加压素诱导的内淋巴积水豚鼠前庭和听觉功能的影响。方法：将60只豚鼠随机均分为四组，每组15只：正常对照组、去氨加压素组、去氨加压素造模联合泽泻汤组、去氨加压素联合双倍剂量泽泻汤组。在第7天和第14天，对每组豚鼠进行骨导颈前庭诱发肌源性电位测试、听性脑干反应和畸变产物耳声发射测试，以定量评估其前庭和听觉功能。功能测试后，通过扫描电子显微镜观察外毛细胞。在第14天，从正常对照组和去氨加压素组中各随机选取1只豚鼠，采用内耳钆增强磁共振成像验证模型的成功建立。
我们对豚鼠进行了骨导颈前庭诱发肌源性电位、听性脑干反应和畸变产物耳声发射测试，结果表明去氨加压素确实影响豚鼠的前庭功能和听力。从具有统计学意义的结果进行分析，泽泻汤能剂量依赖性地改善去氨加压素诱导的前庭功能障碍和听力损失，尽管未实现完全逆转。关于扫描电子显微镜，去氨加压素组外毛细胞显示明显丢失和纤毛倒伏，然而，泽泻汤治疗可减轻外毛细胞丢失和纤毛倒伏。当外毛细胞长时间暴露于去氨加压素时，泽泻汤的改善效果不如以前明显。
改善程度与泽泻汤的浓度和剂量相关。即使剂量加倍，泽泻汤也只能部分减轻去氨加压素诱导的前庭和听觉功能下降，未能完全逆转。
