Bester Rachelle, Goodchild Shannon, Maree Hans J
Department of Genetics, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
Citrus Research International, PO Box 2201, Matieland, 7602, South Africa.
Arch Virol. 2025 Mar 11;170(4):72. doi: 10.1007/s00705-025-06262-0.
The complete genome sequence of fig leaf mottle-associated virus 2 (FLMaV2), a positive-sense single-stranded RNA virus with a genome length of 16,925 nucleotides, was determined via RT-PCR and rapid amplification of cDNA ends (RACE). Its genome organization resembles those of little cherry virus 2 (LChV2) and yam asymptomatic virus 1 (YaV1), both of which are currently classified as members of the genus Ampelovirus but differ from typical ampeloviruses. Phylogenetic analysis based on amino acid sequences of the RNA-dependent RNA polymerase and coat protein of members of the family Closteroviridae showed that FLMaV2 clustered with LChV2 and YaV1. Because of their unusual genome organization, FLMaV2, LChV2, and YaV1 might represent a distinct genus within the family Closteroviridae.
通过逆转录聚合酶链反应（RT-PCR）和cDNA末端快速扩增（RACE）技术，测定了无花果叶斑驳相关病毒2（FLMaV2）的全基因组序列。该病毒为正义单链RNA病毒，基因组长度为16,925个核苷酸。其基因组结构与小樱桃病毒2（LChV2）和山药无症状病毒1（YaV1）相似，后两者目前均被归类为葡萄病毒属成员，但与典型的葡萄病毒不同。基于长线形病毒科成员的RNA依赖RNA聚合酶和外壳蛋白氨基酸序列进行的系统发育分析表明，FLMaV2与LChV2和YaV1聚为一类。由于其独特的基因组结构，FLMaV2、LChV2和YaV1可能代表长线形病毒科内一个独特的属。
